Detailed information of TA system TA088087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3580413..3581038
• Replicon: chromosome
• Accession: NZ_CP024618
• Organism: Escherichia coli strain SMN152SH1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CR916_RS20915
• Protein ID: WP_000911330.1
• Coordinates: 3580413..3580811 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: CR916_RS20920
• Protein ID: WP_000450524.1
• Coordinates: 3580811..3581038 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR916_RS20895	3576387..3576587	+	201	WP_000383836.1	YpfN family protein	-
CR916_RS20900	3576697..3577299	-	603	Protein_3685	esterase	-
CR916_RS20905	3577373..3579388	-	2016	WP_062882060.1	tRNA cytosine(34) acetyltransferase TmcA	-
CR916_RS20910	3579403..3580266	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
CR916_RS20915	3580413..3580811	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CR916_RS20920	3580811..3581038	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CR916_RS20925	3581194..3581907	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CR916_RS20930	3582120..3583154	-	1035	WP_062882059.1	outer membrane protein assembly factor BamC	-
CR916_RS20935	3583171..3584049	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CR916_RS20940	3584195..3584767	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
CR916_RS20945	3584767..3585237	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T88087 WP_000911330.1 NZ_CP024618:c3580811-3580413 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T88087 NZ_CP024618:c3580811-3580413 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT88087 WP_000450524.1 NZ_CP024618:c3581038-3580811 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT88087 NZ_CP024618:c3581038-3580811 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33