Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4066395..4067014 Replicon chromosome
Accession NZ_CP024548
Organism Klebsiella pneumoniae strain KSB1_7J

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CTI66_RS20515 Protein ID WP_002892050.1
Coordinates 4066796..4067014 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CTI66_RS20510 Protein ID WP_002892066.1
Coordinates 4066395..4066769 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CTI66_RS20500 4061547..4062740 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CTI66_RS20505 4062763..4065909 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
CTI66_RS20510 4066395..4066769 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CTI66_RS20515 4066796..4067014 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CTI66_RS20520 4067173..4067739 + 567 WP_002892030.1 maltose O-acetyltransferase -
CTI66_RS27640 4067711..4067851 - 141 WP_004147370.1 hypothetical protein -
CTI66_RS20525 4067872..4068342 + 471 WP_002892026.1 YlaC family protein -
CTI66_RS20530 4068317..4069768 - 1452 WP_021313732.1 PLP-dependent aminotransferase family protein -
CTI66_RS20535 4069869..4070567 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
CTI66_RS20540 4070564..4070704 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CTI66_RS20545 4070704..4070967 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T87950 WP_002892050.1 NZ_CP024548:4066796-4067014 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T87950 NZ_CP024548:4066796-4067014 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT87950 WP_002892066.1 NZ_CP024548:4066395-4066769 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT87950 NZ_CP024548:4066395-4066769 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References