Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 36761..37404 Replicon plasmid unnamed1
Accession NZ_CP024546
Organism Klebsiella pneumoniae strain INF059

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CTI65_RS27070 Protein ID WP_001044770.1
Coordinates 36761..37177 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CTI65_RS27075 Protein ID WP_001261282.1
Coordinates 37174..37404 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CTI65_RS27050 32130..32480 + 351 WP_004187110.1 DUF305 domain-containing protein -
CTI65_RS27055 32666..33580 - 915 WP_031592169.1 HNH endonuclease -
CTI65_RS27060 34118..35140 - 1023 WP_000361404.1 helicase UvrD -
CTI65_RS27065 35125..36687 - 1563 WP_004206609.1 AAA family ATPase -
CTI65_RS27070 36761..37177 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CTI65_RS27075 37174..37404 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CTI65_RS28805 37361..37777 + 417 WP_198406539.1 hypothetical protein -
CTI65_RS27085 37983..38903 + 921 WP_060579408.1 hypothetical protein -
CTI65_RS27090 38900..39709 + 810 WP_099729063.1 hypothetical protein -
CTI65_RS27095 40071..40418 + 348 WP_074460534.1 hypothetical protein -
CTI65_RS27100 40456..41235 + 780 WP_099729064.1 site-specific integrase -
CTI65_RS27105 41321..42352 - 1032 WP_046092923.1 IS630-like element ISEc33 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..110374 110374
- flank IS/Tn - - 41321..42253 932


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T87935 WP_001044770.1 NZ_CP024546:c37177-36761 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T87935 NZ_CP024546:c37177-36761 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT87935 WP_001261282.1 NZ_CP024546:c37404-37174 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT87935 NZ_CP024546:c37404-37174 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References