Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5259568..5260193 Replicon chromosome
Accession NZ_CP024545
Organism Klebsiella pneumoniae strain INF059

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag CTI65_RS26445 Protein ID WP_004187928.1
Coordinates 5259568..5259951 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag CTI65_RS26450 Protein ID WP_004150355.1
Coordinates 5259951..5260193 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CTI65_RS26430 5256934..5257836 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
CTI65_RS26435 5257833..5258468 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
CTI65_RS26440 5258465..5259394 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
CTI65_RS26445 5259568..5259951 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
CTI65_RS26450 5259951..5260193 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
CTI65_RS26455 5260387..5261304 + 918 WP_004187929.1 alpha/beta hydrolase -
CTI65_RS26460 5261318..5262259 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
CTI65_RS26465 5262304..5262741 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
CTI65_RS26470 5262738..5263598 - 861 WP_004146232.1 virulence factor BrkB family protein -
CTI65_RS26475 5263592..5264191 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T87933 WP_004187928.1 NZ_CP024545:c5259951-5259568 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T87933 NZ_CP024545:c5259951-5259568 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTACCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT87933 WP_004150355.1 NZ_CP024545:c5260193-5259951 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT87933 NZ_CP024545:c5260193-5259951 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References