Detailed information of TA system TA087838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2686944..2687563
• Replicon: chromosome
• Accession: NZ_CP024521
• Organism: Klebsiella pneumoniae strain INF158

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: CTI59_RS13520
• Protein ID: WP_002892050.1
• Coordinates: 2687345..2687563 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: CTI59_RS13515
• Protein ID: WP_099595242.1
• Coordinates: 2686944..2687318 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTI59_RS13505	2682096..2683289	+	1194	WP_002892072.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
CTI59_RS13510	2683312..2686458	+	3147	WP_002892069.1	multidrug efflux RND transporter permease subunit AcrB	-
CTI59_RS13515	2686944..2687318	+	375	WP_099595242.1	Hha toxicity modulator TomB	Antitoxin
CTI59_RS13520	2687345..2687563	+	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
CTI59_RS13525	2687722..2688288	+	567	WP_002892030.1	maltose O-acetyltransferase	-
CTI59_RS30400	2688260..2688400	-	141	WP_004147370.1	hypothetical protein	-
CTI59_RS13530	2688421..2688891	+	471	WP_002892026.1	YlaC family protein	-
CTI59_RS13535	2688866..2690317	-	1452	WP_002892023.1	PLP-dependent aminotransferase family protein	-
CTI59_RS13540	2690418..2691116	+	699	WP_002892021.1	GNAT family N-acetyltransferase	-
CTI59_RS13545	2691113..2691253	-	141	WP_002892018.1	type B 50S ribosomal protein L36	-
CTI59_RS13550	2691253..2691516	-	264	WP_002892011.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T87838 WP_002892050.1 NZ_CP024521:2687345-2687563 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T87838 NZ_CP024521:2687345-2687563 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14402.08 Da
• Isoelectric Point: 4.8989

>AT87838 WP_099595242.1 NZ_CP024521:2686944-2687318 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFAFNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT87838 NZ_CP024521:2686944-2687318 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTTTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2