Detailed information of TA system TA087809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 58322..59079
• Replicon: plasmid unnamed1
• Accession: NZ_CP024490
• Organism: Klebsiella pneumoniae strain INF249

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J4ZXV7
• Locus tag: CTI54_RS28605
• Protein ID: WP_029497408.1
• Coordinates: 58597..59079 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: CTI54_RS28600
• Protein ID: WP_094645258.1
• Coordinates: 58322..58609 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTI54_RS28570	53685..53877	-	193	Protein_62	MFS transporter	-
CTI54_RS28575	53907..54884	+	978	WP_004196334.1	chromate resistance protein	-
CTI54_RS28580	54841..56217	+	1377	WP_004196363.1	chromate efflux transporter	-
CTI54_RS28585	56248..56937	-	690	WP_004196322.1	hypothetical protein	-
CTI54_RS28590	56951..57688	-	738	WP_008460272.1	HupE/UreJ family protein	-
CTI54_RS28595	57732..58097	-	366	WP_009651956.1	hypothetical protein	-
CTI54_RS28600	58322..58609	+	288	WP_094645258.1	DUF1778 domain-containing protein	Antitoxin
CTI54_RS28605	58597..59079	+	483	WP_029497408.1	GNAT family N-acetyltransferase	Toxin
CTI54_RS28610	59476..60168	-	693	Protein_70	IS3-like element ISKpn38 family transposase	-
CTI54_RS28615	60198..60554	-	357	Protein_71	transposase	-
CTI54_RS28620	60588..62126	-	1539	WP_004196907.1	IS66-like element ISEc22 family transposase	-
CTI54_RS28625	62175..62522	-	348	WP_004196919.1	IS66 family insertion sequence element accessory protein TnpB	-
CTI54_RS28630	62519..62923	-	405	WP_004196883.1	IS66 family insertion sequence hypothetical protein	-
CTI54_RS28635	63005..63853	-	849	Protein_75	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..71104	71104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17599.34 Da
• Isoelectric Point: 9.8719

>T87809 WP_029497408.1 NZ_CP024490:58597-59079 [Klebsiella pneumoniae]
VGRLTAPEPLSAFHQVAEFVSGEAVLDDWLKQKGLKNQALGAARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFHGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLRLP

Nucleotide

• Length: 483 bp

>T87809 NZ_CP024490:58597-59079 [Klebsiella pneumoniae]
GTGGGACGTTTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTGGCTGAGTTCGTCAGCGGTGAGGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCCCTCGGAGCTGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGCAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGCGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAGCGAACATTGTTTCTTAGACTCCCT
TAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10955.66 Da
• Isoelectric Point: 4.5141

>AT87809 WP_094645258.1 NZ_CP024490:58322-58609 [Klebsiella pneumoniae]
MLYKGCLMKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVVDD
PVIEKLLARKPQWDV

Nucleotide

• Length: 288 bp

>AT87809 NZ_CP024490:58322-58609 [Klebsiella pneumoniae]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAATGGCCTGTCAGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGCGGAGTTCATCGACATGCTTGATGCACCTGTCGTAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTTTAA

Structures

Toxin

• AlphaFold DB: A0A2J4ZXV7