Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 21668..22193 Replicon plasmid unnamed1
Accession NZ_CP024490
Organism Klebsiella pneumoniae strain INF249

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag CTI54_RS28350 Protein ID WP_013023785.1
Coordinates 21888..22193 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag CTI54_RS28345 Protein ID WP_001568025.1
Coordinates 21668..21886 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CTI54_RS28315 17794..18816 - 1023 WP_000361404.1 helicase UvrD -
CTI54_RS28320 18801..20363 - 1563 WP_009309907.1 AAA family ATPase -
CTI54_RS28325 20437..20853 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
CTI54_RS28330 20850..21080 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CTI54_RS29665 21037..21498 + 462 WP_160866775.1 hypothetical protein -
CTI54_RS28345 21668..21886 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CTI54_RS28350 21888..22193 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
CTI54_RS28355 22380..23384 + 1005 WP_029497484.1 hypothetical protein -
CTI54_RS28360 23582..24361 + 780 WP_029497485.1 site-specific integrase -
CTI54_RS28365 24419..24676 - 258 WP_000764642.1 hypothetical protein -
CTI54_RS29670 24805..24918 - 114 WP_012540012.1 hypothetical protein -
CTI54_RS28370 25552..26307 + 756 WP_012539983.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..71104 71104


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T87808 WP_013023785.1 NZ_CP024490:21888-22193 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T87808 NZ_CP024490:21888-22193 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT87808 WP_001568025.1 NZ_CP024490:21668-21886 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT87808 NZ_CP024490:21668-21886 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References