Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5413488..5414113 Replicon chromosome
Accession NZ_CP024489
Organism Klebsiella pneumoniae strain INF249

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag CTI54_RS27330 Protein ID WP_019705794.1
Coordinates 5413488..5413871 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag CTI54_RS27335 Protein ID WP_004150355.1
Coordinates 5413871..5414113 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CTI54_RS27315 5410854..5411756 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
CTI54_RS27320 5411753..5412388 + 636 WP_032430299.1 formate dehydrogenase cytochrome b556 subunit -
CTI54_RS27325 5412385..5413314 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
CTI54_RS27330 5413488..5413871 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
CTI54_RS27335 5413871..5414113 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
CTI54_RS27340 5414318..5415235 + 918 WP_032430300.1 alpha/beta hydrolase -
CTI54_RS27345 5415250..5416191 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
CTI54_RS27350 5416236..5416673 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
CTI54_RS27355 5416670..5417530 - 861 WP_004146232.1 virulence factor BrkB family protein -
CTI54_RS27360 5417524..5418123 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T87806 WP_019705794.1 NZ_CP024489:c5413871-5413488 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T87806 NZ_CP024489:c5413871-5413488 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCATCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT87806 WP_004150355.1 NZ_CP024489:c5414113-5413871 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT87806 NZ_CP024489:c5414113-5413871 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References