Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 5198763..5199499 Replicon chromosome
Accession NZ_CP024489
Organism Klebsiella pneumoniae strain INF249

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag CTI54_RS26245 Protein ID WP_003026803.1
Coordinates 5199017..5199499 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CTI54_RS26240 Protein ID WP_003026799.1
Coordinates 5198763..5199029 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CTI54_RS26195 5194963..5195325 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
CTI54_RS26200 5195375..5195725 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
CTI54_RS26205 5196083..5196331 + 249 WP_019725036.1 hypothetical protein -
CTI54_RS26210 5196328..5196900 + 573 WP_015065504.1 hypothetical protein -
CTI54_RS26215 5196931..5197425 + 495 WP_009310053.1 hypothetical protein -
CTI54_RS26220 5197469..5197837 + 369 WP_015065502.1 hypothetical protein -
CTI54_RS26225 5197871..5198074 + 204 WP_019725040.1 hemolysin expression modulator Hha -
CTI54_RS26230 5198088..5198318 + 231 WP_019725034.1 hypothetical protein -
CTI54_RS29080 5198362..5198556 - 195 WP_019725033.1 hypothetical protein -
CTI54_RS26240 5198763..5199029 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CTI54_RS26245 5199017..5199499 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
CTI54_RS26250 5199707..5201053 + 1347 WP_077253535.1 ISNCY family transposase -
CTI54_RS26255 5201102..5201497 + 396 WP_004143398.1 helix-turn-helix domain-containing protein -
CTI54_RS26260 5201645..5202899 - 1255 Protein_4993 IS3 family transposase -
CTI54_RS26265 5202987..5203949 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 - 5079347..5289591 210244


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T87805 WP_003026803.1 NZ_CP024489:5199017-5199499 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T87805 NZ_CP024489:5199017-5199499 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT87805 WP_003026799.1 NZ_CP024489:5198763-5199029 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT87805 NZ_CP024489:5198763-5199029 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References