Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4032858..4033477 Replicon chromosome
Accession NZ_CP024489
Organism Klebsiella pneumoniae strain INF249

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CTI54_RS20370 Protein ID WP_002892050.1
Coordinates 4033259..4033477 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CTI54_RS20365 Protein ID WP_002892066.1
Coordinates 4032858..4033232 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CTI54_RS20355 4028010..4029203 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CTI54_RS20360 4029226..4032372 + 3147 WP_004147373.1 multidrug efflux RND transporter permease subunit -
CTI54_RS20365 4032858..4033232 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CTI54_RS20370 4033259..4033477 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CTI54_RS20375 4033636..4034202 + 567 WP_002892030.1 maltose O-acetyltransferase -
CTI54_RS29540 4034174..4034314 - 141 WP_004147370.1 hypothetical protein -
CTI54_RS20380 4034335..4034805 + 471 WP_002892026.1 YlaC family protein -
CTI54_RS20385 4034780..4036231 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
CTI54_RS20390 4036332..4037030 + 699 WP_040220001.1 GNAT family N-acetyltransferase -
CTI54_RS20395 4037027..4037167 - 141 WP_040219999.1 type B 50S ribosomal protein L36 -
CTI54_RS20400 4037167..4037430 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T87801 WP_002892050.1 NZ_CP024489:4033259-4033477 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T87801 NZ_CP024489:4033259-4033477 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT87801 WP_002892066.1 NZ_CP024489:4032858-4033232 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT87801 NZ_CP024489:4032858-4033232 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References