Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 736952..737709 Replicon chromosome
Accession NZ_CP024489
Organism Klebsiella pneumoniae strain INF249

Toxin (Protein)


Gene name tacT Uniprot ID A0A1D8JMF4
Locus tag CTI54_RS03790 Protein ID WP_044159004.1
Coordinates 737227..737709 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A1D8JMH3
Locus tag CTI54_RS03785 Protein ID WP_007372584.1
Coordinates 736952..737236 (+) Length 95 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CTI54_RS03765 732344..733066 + 723 WP_013095151.1 TIGR03761 family integrating conjugative element protein -
CTI54_RS03770 733084..735096 + 2013 WP_044159002.1 DNA topoisomerase III -
CTI54_RS03775 735729..736220 + 492 WP_007372582.1 DUF3577 domain-containing protein -
CTI54_RS03780 736296..736796 + 501 WP_007372583.1 single-stranded DNA-binding protein -
CTI54_RS03785 736952..737236 + 285 WP_007372584.1 DUF1778 domain-containing protein Antitoxin
CTI54_RS03790 737227..737709 + 483 WP_044159004.1 GNAT family N-acetyltransferase Toxin
CTI54_RS03795 737942..739228 + 1287 WP_013095156.1 TcpQ domain-containing protein -
CTI54_RS03800 739230..739673 + 444 WP_007372587.1 type IV pilus biogenesis protein PilM -
CTI54_RS03805 739693..741366 + 1674 WP_013095157.1 PilN family type IVB pilus formation outer membrane protein -
CTI54_RS03810 741369..742649 + 1281 WP_176682058.1 type 4b pilus protein PilO2 -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 724822..832258 107436


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(39-146)

Antitoxin

(1-93)


Sequences


Toxin        


Download         Length: 161 a.a.        Molecular weight: 17693.32 Da        Isoelectric Point: 6.9570

>T87790 WP_044159004.1 NZ_CP024489:737227-737709 [Klebsiella pneumoniae]
MEINVTAPALLTEEHVLHSFDCGNAVLDDWLRRRALKNQHLNASRTFVICPEGTACVVGYYSLATGSVSHVDLSRSLRQN
MPDPVPVVLLGRLAVDVSTQGNNFGKWLLNDAVMRVSNLADQVGIKAIMVHAIDERAKAFYEYFGFVQSPVAANTLFYKI

Download         Length: 483 bp

>T87790 NZ_CP024489:737227-737709 [Klebsiella pneumoniae]
ATGGAAATAAATGTCACTGCGCCTGCTTTACTGACAGAGGAGCATGTTCTTCATTCGTTCGATTGCGGGAATGCGGTACT
TGATGACTGGCTTCGCCGGCGTGCGTTGAAGAATCAGCATCTTAATGCTTCCCGGACATTCGTCATCTGTCCTGAAGGTA
CTGCGTGTGTAGTGGGTTATTATTCCCTTGCGACGGGCTCAGTTAGTCATGTCGACTTGAGTCGTAGTCTGCGACAGAAT
ATGCCCGATCCCGTACCGGTTGTTTTGCTGGGCCGCCTTGCAGTCGATGTATCCACTCAGGGCAATAACTTCGGGAAATG
GTTGCTGAATGATGCTGTGATGCGCGTATCGAATCTGGCGGATCAGGTCGGCATCAAGGCTATCATGGTGCATGCAATTG
ATGAGCGGGCTAAAGCGTTCTATGAATACTTTGGTTTTGTGCAGTCACCGGTTGCGGCTAACACGCTTTTCTACAAAATA
TAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 11078.63 Da        Isoelectric Point: 8.5684

>AT87790 WP_007372584.1 NZ_CP024489:736952-737236 [Klebsiella pneumoniae]
MQTPVRKSVRDKQINIRATDEERAVIDYAASLVNKNRTDFIIERAVHEAQNIILDQRVFVLDDARYQAFIRQLEAPVQNV
EGRQRLMDVKPEWK

Download         Length: 285 bp

>AT87790 NZ_CP024489:736952-737236 [Klebsiella pneumoniae]
ATGCAAACTCCAGTCCGTAAATCCGTCCGTGACAAGCAAATCAATATCCGCGCCACGGATGAAGAACGTGCGGTGATTGA
TTACGCTGCGAGTCTGGTTAATAAAAACCGTACTGATTTCATCATTGAAAGGGCTGTGCATGAGGCCCAGAACATCATTC
TGGACCAGCGTGTGTTCGTTCTTGATGATGCCCGTTACCAGGCGTTCATCAGGCAGCTTGAAGCCCCTGTGCAGAATGTT
GAAGGTCGTCAGCGACTGATGGATGTAAAACCTGAATGGAAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A1D8JMF4


Antitoxin

Source ID Structure
AlphaFold DB A0A1D8JMH3

References