Detailed information of TA system TA087781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 34587..35122
• Replicon: plasmid unnamed1
• Accession: NZ_CP024480
• Organism: Escherichia coli strain 2011C-4315

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: A5958_RS28665
• Protein ID: WP_001270421.1
• Coordinates: 34835..35122 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: A5958_RS28660
• Protein ID: WP_001132895.1
• Coordinates: 34587..34838 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5958_RS28625	29795..30100	-	306	WP_032162675.1	hypothetical protein	-
A5958_RS28630	30104..30521	-	418	Protein_34	DUF1281 domain-containing protein	-
A5958_RS28635	30402..31564	+	1163	Protein_35	DUF4113 domain-containing protein	-
A5958_RS28640	31705..32667	-	963	WP_000756329.1	ParB/RepB/Spo0J family partition protein	-
A5958_RS28645	32664..33869	-	1206	WP_000817643.1	AAA family ATPase	-
A5958_RS30010	34240..34413	+	174	WP_001435278.1	hypothetical protein	-
A5958_RS28660	34587..34838	+	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
A5958_RS28665	34835..35122	+	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A5958_RS28670	35124..35529	+	406	Protein_41	DDE-type integrase/transposase/recombinase	-
A5958_RS28680	36175..37152	+	978	WP_000361617.1	RepB family plasmid replication initiator protein	-
A5958_RS28685	37436..38176	-	741	WP_001066926.1	tyrosine-type recombinase/integrase	-
A5958_RS28690	38300..38440	-	141	Protein_44	serine/threonine protein kinase	-
A5958_RS28695	38491..38652	+	162	Protein_45	epoxyqueuosine reductase QueH	-
A5958_RS28700	38963..39337	-	375	WP_001368887.1	hypothetical protein	-
A5958_RS28705	39540..39719	+	180	WP_000526860.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	exeG / exeE / stcE / hlyC / hlyA / hlyB / hlyD	1..77062	77062

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T87781 WP_001270421.1 NZ_CP024480:34835-35122 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T87781 NZ_CP024480:34835-35122 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT87781 WP_001132895.1 NZ_CP024480:34587-34838 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT87781 NZ_CP024480:34587-34838 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTTTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTAGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6