Detailed information of TA system TA087779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4765726..4766351
• Replicon: chromosome
• Accession: NZ_CP024479
• Organism: Escherichia coli strain 2011C-4315

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A5958_RS25555
• Protein ID: WP_000911330.1
• Coordinates: 4765953..4766351 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: A5958_RS25550
• Protein ID: WP_000450524.1
• Coordinates: 4765726..4765953 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5958_RS25525	4761529..4761999	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
A5958_RS25530	4761999..4762571	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
A5958_RS25535	4762717..4763595	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
A5958_RS25540	4763612..4764646	+	1035	WP_001368798.1	outer membrane protein assembly factor BamC	-
A5958_RS25545	4764859..4765572	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
A5958_RS25550	4765726..4765953	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
A5958_RS25555	4765953..4766351	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
A5958_RS25560	4766498..4767361	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
A5958_RS25565	4767376..4769391	+	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
A5958_RS25570	4769465..4770163	+	699	WP_000679812.1	esterase	-
A5958_RS25575	4770273..4770473	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T87779 WP_000911330.1 NZ_CP024479:4765953-4766351 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T87779 NZ_CP024479:4765953-4766351 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT87779 WP_000450524.1 NZ_CP024479:4765726-4765953 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT87779 NZ_CP024479:4765726-4765953 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33