Detailed information of TA system TA087775

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4026438..4027222
• Replicon: chromosome
• Accession: NZ_CP024479
• Organism: Escherichia coli strain 2011C-4315

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: C7BUQ8
• Locus tag: A5958_RS21575
• Protein ID: WP_000854717.1
• Coordinates: 4026438..4026764 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: C7BUQ9
• Locus tag: A5958_RS21580
• Protein ID: WP_001285644.1
• Coordinates: 4026854..4027222 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5958_RS21545	4021733..4022710	+	978	WP_000633206.1	type II secretion system minor pseudopilin GspK	-
A5958_RS21550	4022707..4023885	+	1179	WP_000094966.1	type II secretion system protein GspL	-
A5958_RS21555	4023887..4024423	+	537	WP_000942788.1	GspM family type II secretion system protein YghD	-
A5958_RS21560	4024766..4025608	-	843	WP_001378387.1	DUF4942 domain-containing protein	-
A5958_RS21565	4025693..4025890	-	198	WP_000839243.1	DUF957 domain-containing protein	-
A5958_RS21570	4025902..4026390	-	489	WP_000761729.1	hypothetical protein	-
A5958_RS21575	4026438..4026764	-	327	WP_000854717.1	TA system toxin CbtA family protein	Toxin
A5958_RS21580	4026854..4027222	-	369	WP_001285644.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
A5958_RS21585	4027385..4027606	-	222	WP_000692319.1	DUF987 domain-containing protein	-
A5958_RS21590	4027669..4028145	-	477	WP_001368782.1	RadC family protein	-
A5958_RS21595	4028161..4028634	-	474	WP_001368734.1	antirestriction protein	-
A5958_RS21600	4028728..4028973	-	246	WP_001164975.1	hypothetical protein	-
A5958_RS21605	4028973..4029524	-	552	Protein_3922	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12194.91 Da
• Isoelectric Point: 6.4697

>T87775 WP_000854717.1 NZ_CP024479:c4026764-4026438 [Escherichia coli]
MKTLPDTHVREASRCPSPVIIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDN

Nucleotide

• Length: 327 bp

>T87775 NZ_CP024479:c4026764-4026438 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCATCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACTCGCGA
CAATTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13533.31 Da
• Isoelectric Point: 5.0235

>AT87775 WP_001285644.1 NZ_CP024479:c4027222-4026854 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGVCLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT87775 NZ_CP024479:c4027222-4026854 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGTCTGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: C7BUQ8

Antitoxin

• AlphaFold DB: C7BUQ9