Detailed information of TA system TA087766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1949960..1950797
• Replicon: chromosome
• Accession: NZ_CP024479
• Organism: Escherichia coli strain 2011C-4315

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A0F5GET7
• Locus tag: A5958_RS10960
• Protein ID: WP_000227783.1
• Coordinates: 1950255..1950797 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: A5958_RS10955
• Protein ID: WP_001297137.1
• Coordinates: 1949960..1950271 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5958_RS10930	1944980..1945927	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
A5958_RS10935	1945949..1947940	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
A5958_RS10940	1947930..1948544	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
A5958_RS10945	1948544..1948873	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
A5958_RS10950	1948885..1949775	+	891	WP_000971336.1	heme o synthase	-
A5958_RS10955	1949960..1950271	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
A5958_RS10960	1950255..1950797	+	543	WP_000227783.1	GNAT family N-acetyltransferase	Toxin
A5958_RS10965	1950853..1951788	-	936	WP_001297127.1	sel1 repeat family protein	-
A5958_RS10970	1952196..1953560	+	1365	WP_001000978.1	MFS transporter	-
A5958_RS10975	1953688..1954179	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
A5958_RS10980	1954347..1955258	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19776.96 Da
• Isoelectric Point: 8.0330

>T87766 WP_000227783.1 NZ_CP024479:1950255-1950797 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKQGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T87766 NZ_CP024479:1950255-1950797 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCAGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT87766 WP_001297137.1 NZ_CP024479:1949960-1950271 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT87766 NZ_CP024479:1949960-1950271 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A0F5GET7

Antitoxin

• AlphaFold DB: A0A0E0Y6B3