Detailed information of TA system TA087752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5289378..5289964
• Replicon: chromosome
• Accession: NZ_CP024477
• Organism: Pseudomonas aeruginosa strain 12939

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: CS058_RS25265
• Protein ID: WP_003120987.1
• Coordinates: 5289665..5289964 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: CS058_RS25260
• Protein ID: WP_003448662.1
• Coordinates: 5289378..5289668 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CS058_RS25240	5286356..5287275	+	920	Protein_4891	IS3 family transposase	-
CS058_RS25245	5287302..5288678	+	1377	WP_099887691.1	IS30-like element ISPa37 family transposase	-
CS058_RS25250	5288683..5288973	+	291	Protein_4893	transposase	-
CS058_RS25255	5289023..5289307	+	285	WP_192875560.1	hypothetical protein	-
CS058_RS25260	5289378..5289668	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
CS058_RS25265	5289665..5289964	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CS058_RS25270	5290166..5291290	+	1125	WP_034024019.1	TcpQ domain-containing protein	-
CS058_RS25275	5291290..5292999	+	1710	WP_003123331.1	PilN family type IVB pilus formation outer membrane protein	-
CS058_RS25280	5293003..5294328	+	1326	WP_042180214.1	type 4b pilus protein PilO2	-
CS058_RS25285	5294318..5294851	+	534	WP_023094340.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5256358..5348471	92113

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T87752 WP_003120987.1 NZ_CP024477:c5289964-5289665 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T87752 NZ_CP024477:c5289964-5289665 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT87752 WP_003448662.1 NZ_CP024477:c5289668-5289378 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT87752 NZ_CP024477:c5289668-5289378 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73