Detailed information of TA system TA087751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5278713..5279353
• Replicon: chromosome
• Accession: NZ_CP024477
• Organism: Pseudomonas aeruginosa strain 12939

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CS058_RS25210
• Protein ID: WP_033993319.1
• Coordinates: 5278713..5279123 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6N0KGL5
• Locus tag: CS058_RS25215
• Protein ID: WP_023132185.1
• Coordinates: 5279123..5279353 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CS058_RS25175	5275193..5275921	+	729	WP_099887686.1	TIGR03761 family integrating conjugative element protein	-
CS058_RS25180	5275927..5276475	+	549	WP_099887689.1	DUF3158 family protein	-
CS058_RS25185	5276522..5277361	+	840	WP_003105750.1	Rha family transcriptional regulator	-
CS058_RS25190	5277391..5277879	+	489	WP_004352840.1	single-stranded DNA-binding protein	-
CS058_RS25195	5278035..5278202	+	168	WP_128729268.1	CrpP family protein	-
CS058_RS25200	5278268..5278486	-	219	WP_033993321.1	hypothetical protein	-
CS058_RS25205	5278500..5278697	-	198	WP_003123325.1	hypothetical protein	-
CS058_RS25210	5278713..5279123	-	411	WP_033993319.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CS058_RS25215	5279123..5279353	-	231	WP_023132185.1	antitoxin	Antitoxin
CS058_RS25220	5279609..5281528	+	1920	WP_058149575.1	type I DNA topoisomerase	-
CS058_RS25225	5281835..5282044	+	210	WP_003105733.1	cold-shock protein	-
CS058_RS25230	5282266..5284155	+	1890	WP_016851610.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5256358..5348471	92113

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15422.76 Da
• Isoelectric Point: 7.3233

>T87751 WP_033993319.1 NZ_CP024477:c5279123-5278713 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVAPIV

Nucleotide

• Length: 411 bp

>T87751 NZ_CP024477:c5279123-5278713 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTCGACACCAACATCTGCATATTCACCATCAAAAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGATCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCGGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACT
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGAAAACAAATCGGCCCCTATGACCAGATGATCGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTTGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGATTGGGTCGCCC
CAATCGTGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8436.29 Da
• Isoelectric Point: 4.2821

>AT87751 WP_023132185.1 NZ_CP024477:c5279353-5279123 [Pseudomonas aeruginosa]
VEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTSDFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT87751 NZ_CP024477:c5279353-5279123 [Pseudomonas aeruginosa]
GTGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCAGTCGCGCTTCCCGATGACGT
TAAGCGTGTGGATGTGGTCGCTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGCTGGTTCGATG
GCGAGGGCGTTACTTCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A6N0KGL5