Detailed information of TA system TA087750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 4889821..4890429
• Replicon: chromosome
• Accession: NZ_CP024477
• Organism: Pseudomonas aeruginosa strain 12939

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: Q9I5J9
• Locus tag: CS058_RS23360
• Protein ID: WP_003114156.1
• Coordinates: 4889821..4890168 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: -
• Locus tag: CS058_RS23365
• Protein ID: WP_099887668.1
• Coordinates: 4890178..4890429 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CS058_RS23330	4885180..4885452	+	273	WP_003134390.1	cysteine-rich CWC family protein	-
CS058_RS23335	4885452..4886144	+	693	WP_031636580.1	16S rRNA pseudouridine(516) synthase	-
CS058_RS23340	4886280..4887323	+	1044	WP_003110811.1	L,D-transpeptidase	-
CS058_RS23345	4887403..4888140	+	738	WP_003085453.1	murein L,D-transpeptidase catalytic domain family protein	-
CS058_RS23350	4888592..4889494	+	903	WP_034024280.1	(R)-3-hydroxydecanoyl-ACP:CoA transacylase	-
CS058_RS23360	4889821..4890168	-	348	WP_003114156.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CS058_RS23365	4890178..4890429	-	252	WP_099887668.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CS058_RS23370	4890643..4891626	-	984	WP_034024252.1	tyrosine-type recombinase/integrase	-
CS058_RS23375	4891626..4892918	-	1293	WP_023088869.1	hypothetical protein	-
CS058_RS23380	4893148..4894422	-	1275	WP_099887670.1	zonular occludens toxin family protein	-
CS058_RS23385	4894426..4894782	-	357	WP_003114150.1	DUF2523 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	catB7	-	4889821..4910717	20896

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12984.78 Da
• Isoelectric Point: 4.4212

>T87750 WP_003114156.1 NZ_CP024477:c4890168-4889821 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T87750 NZ_CP024477:c4890168-4889821 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9470.80 Da
• Isoelectric Point: 9.5041

>AT87750 WP_099887668.1 NZ_CP024477:c4890429-4890178 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERRQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT87750 NZ_CP024477:c4890429-4890178 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGATCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAGGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Structures

Toxin

• AlphaFold DB: Q9I5J9