Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 195812..196569 Replicon plasmid unnamed3
Accession NZ_CP024476
Organism Shigella flexneri 7b strain 94-3007

Toxin (Protein)


Gene name tacT Uniprot ID D2AJG5
Locus tag CPZ98_RS25540 Protein ID WP_000501974.1
Coordinates 196084..196569 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag CPZ98_RS25535 Protein ID WP_011114751.1
Coordinates 195812..196096 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ98_RS25500 191873..192547 + 675 WP_001088287.1 IS66-like element accessory protein TnpA -
CPZ98_RS25505 192544..192894 + 351 WP_005048975.1 IS66 family insertion sequence element accessory protein TnpB -
CPZ98_RS25510 192891..194492 + 1602 WP_005063916.1 IS66-like element ISSfl3 family transposase -
CPZ98_RS26165 194746..194910 - 165 WP_001346193.1 hypothetical protein -
CPZ98_RS25535 195812..196096 + 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
CPZ98_RS25540 196084..196569 + 486 WP_000501974.1 GNAT family N-acetyltransferase Toxin
CPZ98_RS25545 196742..197023 - 282 Protein_233 IS4/IS5 family transposase -
CPZ98_RS25550 197090..198246 + 1157 WP_134796931.1 IS3-like element IS600 family transposase -
CPZ98_RS25555 198360..199003 + 644 Protein_235 IS3-like element IS2 family transposase -
CPZ98_RS25565 200268..200840 + 573 Protein_237 IS3-like element IS2 family transposase -
CPZ98_RS25570 200878..201021 + 144 Protein_238 DUF3440 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ipaH7.8 / ospC2 / ipaH2.5 / ospE2 / ipgB2 / ospD1 / ospF / ospD2 / ospB / icsP/sopA / nleE / ospE1 / ipaH1.4 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospC1 / ospD3/senA / ipaH4.5 1..220282 220282
- inside IScluster/Tn - ospC3 / ospC1 / ospD3/senA 183883..219065 35182


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17814.63 Da        Isoelectric Point: 9.8822

>T87744 WP_000501974.1 NZ_CP024476:196084-196569 [Shigella flexneri 7b]
MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T87744 NZ_CP024476:196084-196569 [Shigella flexneri 7b]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCAGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGAGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT87744 WP_011114751.1 NZ_CP024476:195812-196096 [Shigella flexneri 7b]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT87744 NZ_CP024476:195812-196096 [Shigella flexneri 7b]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PN28

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References