Detailed information of TA system TA087722

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3544287..3544813
• Replicon: chromosome
• Accession: NZ_CP024473
• Organism: Shigella flexneri 7b strain 94-3007

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: CPZ98_RS18020
• Protein ID: WP_000323025.1
• Coordinates: 3544526..3544813 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: CPZ98_RS18015
• Protein ID: WP_000534858.1
• Coordinates: 3544287..3544526 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ98_RS17995 (3541882)	3541882..3542310	-	429	Protein_3461	fimbrial protein	-
CPZ98_RS18000 (3542367)	3542367..3543064	+	698	WP_232046037.1	IS1 family transposase	-
CPZ98_RS18005 (3543082)	3543082..3543960	+	879	Protein_3463	IS3-like element IS600 family transposase	-
CPZ98_RS18010 (3543957)	3543957..3544262	-	306	WP_071818640.1	hypothetical protein	-
CPZ98_RS18015 (3544287)	3544287..3544526	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
CPZ98_RS18020 (3544526)	3544526..3544813	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
CPZ98_RS18025 (3544885)	3544885..3545040	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
CPZ98_RS18030 (3545258)	3545258..3545473	+	216	WP_000980986.1	hypothetical protein	-
CPZ98_RS18035 (3545576)	3545576..3545854	+	279	Protein_3469	hypothetical protein	-
CPZ98_RS18040 (3545856)	3545856..3546905	+	1050	WP_001265249.1	DUF968 domain-containing protein	-
CPZ98_RS18045 (3546918)	3546918..3547274	+	357	WP_000904111.1	RusA family crossover junction endodeoxyribonuclease	-
CPZ98_RS18050 (3547289)	3547289..3548110	+	822	WP_000762884.1	antitermination protein	-
CPZ98_RS18070 (3549031)	3549031..3549139	+	109	Protein_3473	DUF3927 family protein	-
CPZ98_RS18075 (3549420)	3549420..3549755	-	336	WP_000871291.1	anti-adapter protein IraM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3536923..3561249	24326

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T87722 WP_000323025.1 NZ_CP024473:3544526-3544813 [Shigella flexneri 7b]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T87722 NZ_CP024473:3544526-3544813 [Shigella flexneri 7b]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT87722 WP_000534858.1 NZ_CP024473:3544287-3544526 [Shigella flexneri 7b]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT87722 NZ_CP024473:3544287-3544526 [Shigella flexneri 7b]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8