Detailed information of TA system TA087710

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 973834..974465
• Replicon: chromosome
• Accession: NZ_CP024473
• Organism: Shigella flexneri 7b strain 94-3007

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CPZ98_RS04925
• Protein ID: WP_025760846.1
• Coordinates: 974190..974465 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: CPZ98_RS04920
• Protein ID: WP_000593555.1
• Coordinates: 973834..974193 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ98_RS04895 (969966)	969966..970910	+	945	WP_000947076.1	nickel ABC transporter permease subunit NikB	-
CPZ98_RS04900 (970907)	970907..971740	+	834	WP_001008974.1	nickel ABC transporter permease subunit NikC	-
CPZ98_RS04905 (971740)	971740..972504	+	765	WP_001136248.1	nickel import ATP-binding protein NikD	-
CPZ98_RS04910 (972501)	972501..973307	+	807	WP_000173660.1	nickel import ATP-binding protein NikE	-
CPZ98_RS04915 (973313)	973313..973714	+	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
CPZ98_RS04920 (973834)	973834..974193	-	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CPZ98_RS04925 (974190)	974190..974465	-	276	WP_025760846.1	type II toxin-antitoxin system HicA family toxin	Toxin
CPZ98_RS04930 (974524)	974524..975648	-	1125	WP_001216257.1	ABC transporter permease	-
CPZ98_RS04935 (975648)	975648..978383	-	2736	WP_000149172.1	ribosome-associated ATPase/putative transporter RbbA	-
CPZ98_RS04940 (978380)	978380..979447	-	1068	WP_000361470.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-81)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10145.95 Da
• Isoelectric Point: 10.6469

>T87710 WP_025760846.1 NZ_CP024473:c974465-974190 [Shigella flexneri 7b]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKKGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T87710 NZ_CP024473:c974465-974190 [Shigella flexneri 7b]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGAAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT87710 WP_000593555.1 NZ_CP024473:c974193-973834 [Shigella flexneri 7b]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT87710 NZ_CP024473:c974193-973834 [Shigella flexneri 7b]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6