Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 160277..160902 Replicon plasmid unnamed3
Accession NZ_CP024469
Organism Shigella dysenteriae strain BU53M1

Toxin (Protein)


Gene name vapC Uniprot ID A0A090N9J2
Locus tag CPZ97_RS26415 Protein ID WP_000911312.1
Coordinates 160277..160675 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q326X5
Locus tag CPZ97_RS26420 Protein ID WP_000450529.1
Coordinates 160675..160902 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ97_RS28120 155594..155725 + 132 Protein_187 transposase -
CPZ97_RS26395 155870..156292 - 423 Protein_188 hypothetical protein -
CPZ97_RS26400 156465..157223 + 759 Protein_189 IS4 family transposase -
CPZ97_RS26410 158486..160268 + 1783 Protein_191 type IV conjugative transfer system coupling protein TraD -
CPZ97_RS26415 160277..160675 - 399 WP_000911312.1 tRNA(fMet)-specific endonuclease VapC Toxin
CPZ97_RS26420 160675..160902 - 228 WP_000450529.1 toxin-antitoxin system antitoxin VapB Antitoxin
CPZ97_RS26425 160987..161403 + 417 WP_171547753.1 conjugative relaxase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / nleE / icsP/sopA / ospB / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ipaH4.5 / ipaH7.8 / ipaH1.4 / ospE2 / ospC1 / ospD3/senA / ospC2 / ospI / ipaH9.8 / ospG / ospC3 1..184894 184894


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14869.20 Da        Isoelectric Point: 8.5264

>T87706 WP_000911312.1 NZ_CP024469:c160675-160277 [Shigella dysenteriae]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAAIHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Download         Length: 399 bp

>T87706 NZ_CP024469:c160675-160277 [Shigella dysenteriae]
ATGCTGAAGTTTATGCTCGATACCAACATCTGTATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTTAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCCGCTGCAGCCATACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAACAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8593.63 Da        Isoelectric Point: 4.5680

>AT87706 WP_000450529.1 NZ_CP024469:c160902-160675 [Shigella dysenteriae]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPDMQERESF

Download         Length: 228 bp

>AT87706 NZ_CP024469:c160902-160675 [Shigella dysenteriae]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGACATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090N9J2

Antitoxin

Source ID Structure
AlphaFold DB A0A090NVK5

References