Detailed information of TA system TA087699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 18569..19091
• Replicon: plasmid unnamed1
• Accession: NZ_CP024467
• Organism: Shigella dysenteriae strain BU53M1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D0QMR1
• Locus tag: CPZ97_RS24350
• Protein ID: WP_000220561.1
• Coordinates: 18810..19091 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: CPZ97_RS24345
• Protein ID: WP_000121743.1
• Coordinates: 18569..18820 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ97_RS24315	13715..14692	+	978	WP_073559672.1	hypothetical protein	-
CPZ97_RS24320	14689..15114	-	426	WP_000202958.1	hypothetical protein	-
CPZ97_RS24325	15185..15628	-	444	WP_001074379.1	hypothetical protein	-
CPZ97_RS24330	15632..16474	-	843	WP_073559670.1	replication initiation protein	-
CPZ97_RS24335	16495..17334	-	840	WP_001352874.1	replication initiation protein	-
CPZ97_RS24345	18569..18820	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
CPZ97_RS24350	18810..19091	+	282	WP_000220561.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CPZ97_RS24355	19237..19560	+	324	WP_001181905.1	hypothetical protein	-
CPZ97_RS24360	19608..19853	+	246	WP_052909233.1	hypothetical protein	-
CPZ97_RS24365	19843..20058	+	216	WP_001180116.1	hypothetical protein	-
CPZ97_RS24370	20151..20480	+	330	WP_000866648.1	hypothetical protein	-
CPZ97_RS24375	20523..20702	+	180	WP_072104066.1	hypothetical protein	-
CPZ97_RS24380	20724..20921	+	198	WP_001675595.1	hypothetical protein	-
CPZ97_RS24385	20934..21206	+	273	WP_000160399.1	hypothetical protein	-
CPZ97_RS28085	21463..21537	+	75	Protein_26	DNA distortion polypeptide 3	-
CPZ97_RS24390	21532..22077	+	546	WP_000757693.1	hypothetical protein	-
CPZ97_RS24395	22080..23246	+	1167	WP_052909232.1	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	catA1 / blaTEM-1D	-	1..54993	54993

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11023.86 Da
• Isoelectric Point: 10.5938

>T87699 WP_000220561.1 NZ_CP024467:18810-19091 [Shigella dysenteriae]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Nucleotide

• Length: 282 bp

>T87699 NZ_CP024467:18810-19091 [Shigella dysenteriae]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTTGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT87699 WP_000121743.1 NZ_CP024467:18569-18820 [Shigella dysenteriae]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT87699 NZ_CP024467:18569-18820 [Shigella dysenteriae]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: A0A3S5I302

Antitoxin

• AlphaFold DB: A0A3S5I301