Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3347891..3348645 Replicon chromosome
Accession NZ_CP024466
Organism Shigella dysenteriae strain BU53M1

Toxin (Protein)


Gene name ataT Uniprot ID Q32AN3
Locus tag CPZ97_RS18540 Protein ID WP_000513068.1
Coordinates 3347891..3348376 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A2X2KZA0
Locus tag CPZ97_RS18545 Protein ID WP_000801913.1
Coordinates 3348367..3348645 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ97_RS18510 3343842..3344168 + 327 WP_000371932.1 thiosulfate sulfurtransferase GlpE -
CPZ97_RS18515 3344213..3345043 + 831 WP_000928722.1 rhomboid family intramembrane serine protease GlpG -
CPZ97_RS18520 3345060..3345818 + 759 WP_000815099.1 DeoR/GlpR family transcriptional regulator -
CPZ97_RS18525 3345800..3346309 - 510 Protein_3510 DNA-binding transcriptional regulator RtcR -
CPZ97_RS18530 3346309..3346809 + 501 Protein_3511 RNA-splicing ligase RtcB -
CPZ97_RS18535 3346813..3347841 + 1029 WP_024259430.1 RNA 3'-terminal phosphate cyclase -
CPZ97_RS18540 3347891..3348376 - 486 WP_000513068.1 GNAT family N-acetyltransferase Toxin
CPZ97_RS18545 3348367..3348645 - 279 WP_000801913.1 DUF1778 domain-containing protein Antitoxin
CPZ97_RS18550 3348712..3351411 - 2700 WP_134802126.1 HTH-type transcriptional regulator MalT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3333423..3375841 42418


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-143)

Antitoxin

Vibrio phage ICP1, Orf50

(2-92)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17631.53 Da        Isoelectric Point: 9.1197

>T87697 WP_000513068.1 NZ_CP024466:c3348376-3347891 [Shigella dysenteriae]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRHNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Download         Length: 486 bp

>T87697 NZ_CP024466:c3348376-3347891 [Shigella dysenteriae]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCATTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACATAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10449.00 Da        Isoelectric Point: 4.6336

>AT87697 WP_000801913.1 NZ_CP024466:c3348645-3348367 [Shigella dysenteriae]
MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQVK
INALMNRKSPWE

Download         Length: 279 bp

>AT87697 NZ_CP024466:c3348645-3348367 [Shigella dysenteriae]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGTCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090ND96

Antitoxin

Source ID Structure
AlphaFold DB A0A2X2KZA0

References