Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2869760..2870414 Replicon chromosome
Accession NZ_CP024466
Organism Shigella dysenteriae strain BU53M1

Toxin (Protein)


Gene name cptA Uniprot ID Q32BV9
Locus tag CPZ97_RS15860 Protein ID WP_000244779.1
Coordinates 2869760..2870167 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CPZ97_RS15865 Protein ID WP_000354046.1
Coordinates 2870148..2870414 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ97_RS15840 2865717..2867450 - 1734 WP_134802106.1 single-stranded-DNA-specific exonuclease RecJ -
CPZ97_RS15845 2867456..2868166 - 711 WP_000715209.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CPZ97_RS15850 2868191..2869087 - 897 WP_078232661.1 site-specific tyrosine recombinase XerD -
CPZ97_RS15855 2869199..2869720 + 522 WP_001055874.1 flavodoxin FldB -
CPZ97_RS15860 2869760..2870167 - 408 WP_000244779.1 protein YgfX Toxin
CPZ97_RS15865 2870148..2870414 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CPZ97_RS15870 2870657..2871637 + 981 WP_000886092.1 tRNA-modifying protein YgfZ -
CPZ97_RS15875 2871714..2872310 - 597 Protein_3005 hemolysin III family protein -
CPZ97_RS15880 2872368..2873062 + 695 WP_087786203.1 IS1-like element IS1N family transposase -
CPZ97_RS15885 2873311..2873622 - 312 WP_134802107.1 N(4)-acetylcytidine aminohydrolase -
CPZ97_RS15890 2873667..2875100 + 1434 Protein_3008 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2872667..2873062 395


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.2669

>T87696 WP_000244779.1 NZ_CP024466:c2870167-2869760 [Shigella dysenteriae]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRWILLQQETQR

Download         Length: 408 bp

>T87696 NZ_CP024466:c2870167-2869760 [Shigella dysenteriae]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGTGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT87696 WP_000354046.1 NZ_CP024466:c2870414-2870148 [Shigella dysenteriae]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT87696 NZ_CP024466:c2870414-2870148 [Shigella dysenteriae]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTCAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NWM4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References