Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 392757..393375 Replicon chromosome
Accession NZ_CP024466
Organism Shigella dysenteriae strain BU53M1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CPZ97_RS02030 Protein ID WP_001291435.1
Coordinates 393157..393375 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CPZ97_RS02025 Protein ID WP_000344800.1
Coordinates 392757..393131 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ97_RS02015 387846..389039 + 1194 WP_005019446.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CPZ97_RS02020 389062..392211 + 3150 WP_134801981.1 efflux RND transporter permease AcrB -
CPZ97_RS02025 392757..393131 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CPZ97_RS02030 393157..393375 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CPZ97_RS02035 393547..394098 + 552 WP_000102564.1 maltose O-acetyltransferase -
CPZ97_RS02040 394214..394684 + 471 WP_000136192.1 YlaC family protein -
CPZ97_RS02045 394848..395048 + 201 WP_134801982.1 hypothetical protein -
CPZ97_RS02050 395137..396365 + 1229 WP_088895425.1 IS3-like element IS2 family transposase -
CPZ97_RS02055 396431..397128 - 698 Protein_392 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T87684 WP_001291435.1 NZ_CP024466:393157-393375 [Shigella dysenteriae]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T87684 NZ_CP024466:393157-393375 [Shigella dysenteriae]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT87684 WP_000344800.1 NZ_CP024466:392757-393131 [Shigella dysenteriae]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT87684 NZ_CP024466:392757-393131 [Shigella dysenteriae]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References