Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4525..5117 Replicon plasmid pMR0617mcr1
Accession NZ_CP024462
Organism Klebsiella pneumoniae strain QS17-0161

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag CR231_RS28545 Protein ID WP_000520549.1
Coordinates 4525..4773 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag CR231_RS28550 Protein ID WP_000681613.1
Coordinates 4770..5117 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR231_RS29065 1402..1917 + 516 WP_001025395.1 J domain-containing protein -
CR231_RS28515 2022..2564 + 543 WP_001083906.1 hypothetical protein -
CR231_RS28520 2561..2812 + 252 WP_001230707.1 hypothetical protein -
CR231_RS28525 2799..3026 + 228 WP_000801440.1 hypothetical protein -
CR231_RS28535 3480..3779 + 300 WP_000650309.1 hypothetical protein -
CR231_RS28540 3870..4220 + 351 WP_000854259.1 hypothetical protein -
CR231_RS28545 4525..4773 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
CR231_RS28550 4770..5117 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CR231_RS28560 5478..5981 + 504 WP_000781813.1 hypothetical protein -
CR231_RS28565 5985..7214 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
CR231_RS28570 7291..7584 + 294 WP_000134733.1 LuxR family transcriptional regulator -
CR231_RS28575 7581..7796 + 216 WP_000760375.1 EexN family lipoprotein -
CR231_RS28580 8152..8772 + 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
CR231_RS28585 8840..9112 + 273 WP_001328551.1 TrbC/VirB2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33858 33858


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T87681 WP_000520549.1 NZ_CP024462:4525-4773 [Klebsiella pneumoniae]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T87681 NZ_CP024462:4525-4773 [Klebsiella pneumoniae]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT87681 WP_000681613.1 NZ_CP024462:4770-5117 [Klebsiella pneumoniae]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT87681 NZ_CP024462:4770-5117 [Klebsiella pneumoniae]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References