Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 51950..52686 Replicon plasmid pMR0617aac
Accession NZ_CP024459
Organism Klebsiella pneumoniae strain QS17-0161

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag CR231_RS26540 Protein ID WP_003026803.1
Coordinates 52204..52686 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CR231_RS26535 Protein ID WP_003026799.1
Coordinates 51950..52216 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR231_RS29020 47034..47483 + 450 WP_105910642.1 hypothetical protein -
CR231_RS29595 47601..48788 + 1188 Protein_51 ISL3 family transposase -
CR231_RS26510 48777..50240 + 1464 Protein_52 group II intron reverse transcriptase/maturase -
CR231_RS26515 50472..50702 + 231 WP_004118481.1 hypothetical protein -
CR231_RS26525 51225..51527 + 303 Protein_54 antirestriction protein -
CR231_RS26530 51481..51741 + 261 Protein_55 hypothetical protein -
CR231_RS26535 51950..52216 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CR231_RS26540 52204..52686 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
CR231_RS26545 52887..54290 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
CR231_RS26550 54319..54951 - 633 WP_001567369.1 hypothetical protein -
CR231_RS26555 55181..56527 + 1347 WP_020314316.1 ISNCY family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / aac(3)-IIa vat / cseA / iutA / iucD / iucC / iucB / iucA 1..211313 211313
- inside IScluster/Tn - cseA 36591..62054 25463


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T87679 WP_003026803.1 NZ_CP024459:52204-52686 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T87679 NZ_CP024459:52204-52686 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT87679 WP_003026799.1 NZ_CP024459:51950-52216 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT87679 NZ_CP024459:51950-52216 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References