Detailed information of TA system TA087678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 34793..35436
• Replicon: plasmid pMR0617aac
• Accession: NZ_CP024459
• Organism: Klebsiella pneumoniae strain QS17-0161

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A6H2GRP8
• Locus tag: CR231_RS26430
• Protein ID: WP_023292131.1
• Coordinates: 35020..35436 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6B7Q5S4
• Locus tag: CR231_RS26425
• Protein ID: WP_021312476.1
• Coordinates: 34793..35023 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR231_RS29590	31603..31889	+	287	Protein_30	hypothetical protein	-
CR231_RS26405	31899..32213	+	315	WP_065801600.1	hypothetical protein	-
CR231_RS26410	32264..33610	-	1347	WP_072123333.1	hypothetical protein	-
CR231_RS26415	33909..34421	+	513	WP_065801590.1	hypothetical protein	-
CR231_RS26425	34793..35023	+	231	WP_021312476.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CR231_RS26430	35020..35436	+	417	WP_023292131.1	type II toxin-antitoxin system VapC family toxin	Toxin
CR231_RS26435	35471..35716	+	246	WP_065801591.1	hypothetical protein	-
CR231_RS26440	35780..36034	+	255	WP_065801592.1	hypothetical protein	-
CR231_RS26450	36591..37597	+	1007	Protein_38	IS110 family transposase	-
CR231_RS26455	37876..38124	+	249	WP_065801593.1	hypothetical protein	-
CR231_RS26460	38129..38701	+	573	WP_099751958.1	hypothetical protein	-
CR231_RS26465	38732..39127	+	396	Protein_41	DNA-binding protein	-
CR231_RS26470	39118..39243	+	126	Protein_42	IS481 family transposase	-
CR231_RS26475	39374..40204	-	831	WP_074425395.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / aac(3)-IIa	vat / cseA / iutA / iucD / iucC / iucB / iucA	1..211313	211313
	-	inside	IScluster/Tn	-	cseA	36591..62054	25463

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14975.40 Da
• Isoelectric Point: 8.5464

>T87678 WP_023292131.1 NZ_CP024459:35020-35436 [Klebsiella pneumoniae]
VKKTFMLDTNICSFIMREQPAAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVN

Nucleotide

• Length: 417 bp

>T87678 NZ_CP024459:35020-35436 [Klebsiella pneumoniae]
GTGAAAAAGACTTTTATGCTCGACACAAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGTGGCCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCCCCGCGCCATATCGAGCTGGTTGACGCGTTCTGTGCCCGCCTCGATGCCATCCTGCCCTGGGACCGAGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCCTTGCGACAGGCCGGGACGCCGATTGGCCCGAACGACACGGCGATAGC
CGGGCACGCCATCGCGGCCGGCGCCGTTCTGGTGACGAATAATGTAAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8723.76 Da
• Isoelectric Point: 4.3410

>AT87678 WP_021312476.1 NZ_CP024459:34793-35023 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT87678 NZ_CP024459:34793-35023 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGTGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGCGAGGATGTGGTCAGCGACGAAGGACGATTTGACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A6H2GRP8

Antitoxin

• AlphaFold DB: A0A6B7Q5S4