Detailed information of TA system TA087663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 707064..707683
• Replicon: chromosome
• Accession: NZ_CP024458
• Organism: Klebsiella pneumoniae strain QS17-0161

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: CR231_RS03585
• Protein ID: WP_002892050.1
• Coordinates: 707064..707282 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: CR231_RS03590
• Protein ID: WP_080789174.1
• Coordinates: 707309..707683 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR231_RS03555	703112..703375	+	264	WP_002892011.1	type B 50S ribosomal protein L31	-
CR231_RS03560	703375..703515	+	141	WP_002892018.1	type B 50S ribosomal protein L36	-
CR231_RS03565	703512..704210	-	699	WP_002892021.1	GNAT family N-acetyltransferase	-
CR231_RS03570	704355..705761	+	1407	WP_020316531.1	PLP-dependent aminotransferase family protein	-
CR231_RS03575	705736..706206	-	471	WP_002892026.1	YlaC family protein	-
CR231_RS29130	706227..706367	+	141	WP_004147370.1	hypothetical protein	-
CR231_RS03580	706339..706905	-	567	WP_002892030.1	maltose O-acetyltransferase	-
CR231_RS03585	707064..707282	-	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
CR231_RS03590	707309..707683	-	375	WP_080789174.1	Hha toxicity modulator TomB	Antitoxin
CR231_RS03595	708169..711315	-	3147	WP_002892069.1	multidrug efflux RND transporter permease subunit AcrB	-
CR231_RS03600	711338..712531	-	1194	WP_004177236.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T87663 WP_002892050.1 NZ_CP024458:c707282-707064 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T87663 NZ_CP024458:c707282-707064 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14295.99 Da
• Isoelectric Point: 5.1444

>AT87663 WP_080789174.1 NZ_CP024458:c707683-707309 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAGDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT87663 NZ_CP024458:c707683-707309 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGGGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2