Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 20851..21374 Replicon plasmid p48896_2
Accession NZ_CP024431
Organism Klebsiella pneumoniae strain DA48896

Toxin (Protein)


Gene name ccdB Uniprot ID V5RNQ6
Locus tag BKL84_RS28080 Protein ID WP_015632464.1
Coordinates 20851..21156 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V5RQR8
Locus tag BKL84_RS28085 Protein ID WP_015632465.1
Coordinates 21156..21374 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKL84_RS28035 15881..16309 - 429 WP_015632456.1 plasmid partitioning/stability family protein -
BKL84_RS28040 16302..17282 - 981 WP_015632457.1 partitioning protein -
BKL84_RS28045 17643..17825 + 183 WP_015632458.1 type II toxin-antitoxin system HicA family toxin -
BKL84_RS28050 17851..18294 + 444 WP_015632459.1 type II toxin-antitoxin system HicB family antitoxin -
BKL84_RS28055 18500..18622 - 123 Protein_25 transcriptional regulator -
BKL84_RS28060 18636..18863 - 228 WP_016162072.1 hypothetical protein -
BKL84_RS28065 19293..19550 + 258 WP_016162071.1 helix-turn-helix domain-containing protein -
BKL84_RS28070 19754..20320 - 567 WP_015632462.1 hypothetical protein -
BKL84_RS28075 20362..20706 - 345 WP_015632463.1 hypothetical protein -
BKL84_RS28080 20851..21156 - 306 WP_015632464.1 type II toxin-antitoxin system toxin CcdB Toxin
BKL84_RS28085 21156..21374 - 219 WP_015632465.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BKL84_RS28090 21560..22585 + 1026 WP_015632466.1 IS110 family transposase -
BKL84_RS28095 22880..23365 + 486 Protein_33 IS5 family transposase -
BKL84_RS28100 23529..23960 + 432 WP_015632467.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
BKL84_RS28105 23960..25231 + 1272 WP_016162068.1 Y-family DNA polymerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid rmtF - 1..114815 114815


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11361.90 Da        Isoelectric Point: 5.5252

>T87656 WP_015632464.1 NZ_CP024431:c21156-20851 [Klebsiella pneumoniae]
MQFTVYEYTRESHYRLFVDVQSDIVETPGRRIVIPLLESHHLSEKVNQQLFPVVSVNGKEYRAVTSELSGVSVSVIGDAV
ADLSHEANAIKNALNLMFWGI

Download         Length: 306 bp

>T87656 NZ_CP024431:c21156-20851 [Klebsiella pneumoniae]
ATGCAGTTCACCGTCTACGAATACACACGCGAGAGCCATTATCGTCTTTTTGTGGATGTGCAGAGCGACATTGTCGAGAC
GCCTGGGCGACGCATAGTGATCCCGTTGCTGGAATCGCACCACTTATCAGAAAAGGTTAATCAGCAGCTTTTCCCGGTGG
TTAGCGTCAATGGGAAAGAATACCGTGCCGTGACTTCCGAATTATCCGGAGTCTCTGTCAGTGTCATCGGCGACGCTGTG
GCCGATCTCAGCCATGAGGCAAATGCCATTAAAAATGCCCTGAACCTGATGTTCTGGGGCATTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8194.13 Da        Isoelectric Point: 6.2224

>AT87656 WP_015632465.1 NZ_CP024431:c21374-21156 [Klebsiella pneumoniae]
MKHRASVTVDKEHYLELLAAGVNISGLVNEAIGREARRIQAERWKAENREGMEEVANFIAQHGSFADENRSW

Download         Length: 219 bp

>AT87656 NZ_CP024431:c21374-21156 [Klebsiella pneumoniae]
ATGAAACACAGAGCCAGCGTCACAGTAGACAAGGAACATTATCTGGAATTACTCGCGGCAGGCGTGAATATTTCGGGACT
GGTGAATGAGGCTATCGGACGGGAAGCCCGGCGCATCCAGGCTGAACGCTGGAAAGCAGAGAACCGGGAAGGGATGGAAG
AGGTAGCGAATTTCATCGCGCAGCACGGCTCTTTTGCCGACGAAAACCGGAGCTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0ND19

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0L1T7

References