Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 22916..23559 Replicon plasmid p48896_1
Accession NZ_CP024430
Organism Klebsiella pneumoniae strain DA48896

Toxin (Protein)


Gene name vagD Uniprot ID R9WRW3
Locus tag BKL84_RS27200 Protein ID WP_015063455.1
Coordinates 22916..23332 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag BKL84_RS27205 Protein ID WP_001261276.1
Coordinates 23329..23559 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKL84_RS27175 18304..19860 + 1557 WP_053897648.1 PAS domain-containing methyl-accepting chemotaxis protein -
BKL84_RS27180 20163..20441 - 279 Protein_21 IS3 family transposase -
BKL84_RS27185 20458..21534 - 1077 WP_000227969.1 IS110 family transposase -
BKL84_RS27195 21816..22672 - 857 Protein_23 IS3-like element ISEc15 family transposase -
BKL84_RS27200 22916..23332 - 417 WP_015063455.1 type II toxin-antitoxin system VapC family toxin Toxin
BKL84_RS27205 23329..23559 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BKL84_RS27215 24133..24483 + 351 WP_000493378.1 hypothetical protein -
BKL84_RS27220 24534..25277 + 744 WP_000129823.1 hypothetical protein -
BKL84_RS27225 25274..26050 + 777 WP_000015958.1 site-specific integrase -
BKL84_RS27230 26108..26365 - 258 WP_000764642.1 hypothetical protein -
BKL84_RS30065 26494..26598 - 105 WP_032409716.1 hypothetical protein -
BKL84_RS27235 27133..27999 + 867 WP_004118283.1 replication initiation protein -
BKL84_RS27240 28176..28445 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-15 / aadA2 / dfrA12 / catA2 - 1..131243 131243


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15036.50 Da        Isoelectric Point: 8.5440

>T87651 WP_015063455.1 NZ_CP024430:c23332-22916 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLVLEDWVN

Download         Length: 417 bp

>T87651 NZ_CP024430:c23332-22916 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCGTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT87651 WP_001261276.1 NZ_CP024430:c23559-23329 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT87651 NZ_CP024430:c23559-23329 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGTGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGCGAGGATGTGGTCAGCGACGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R9WRW3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References