Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 3849828..3850471 Replicon chromosome
Accession NZ_CP024429
Organism Klebsiella pneumoniae strain DA48896

Toxin (Protein)


Gene name vagD Uniprot ID A0A4S8C081
Locus tag BKL84_RS19185 Protein ID WP_032433387.1
Coordinates 3849828..3850244 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag BKL84_RS19190 Protein ID WP_001261276.1
Coordinates 3850241..3850471 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKL84_RS19155 3844997..3845200 - 204 WP_032433376.1 hemolysin expression modulator Hha -
BKL84_RS19160 3845260..3845751 - 492 WP_032433378.1 hypothetical protein -
BKL84_RS19165 3845782..3846300 - 519 WP_045326794.1 hypothetical protein -
BKL84_RS19170 3846359..3846607 - 249 WP_032433382.1 hypothetical protein -
BKL84_RS19175 3846926..3848497 + 1572 WP_032433383.1 ATP-binding protein -
BKL84_RS19180 3848692..3849672 - 981 WP_032433385.1 hypothetical protein -
BKL84_RS19185 3849828..3850244 - 417 WP_032433387.1 type II toxin-antitoxin system VapC family toxin Toxin
BKL84_RS19190 3850241..3850471 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BKL84_RS19200 3850776..3851591 + 816 WP_032433388.1 hypothetical protein -
BKL84_RS19210 3852119..3854359 - 2241 WP_045326788.1 NTPase -
BKL84_RS19215 3854362..3855449 - 1088 Protein_3631 transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3822101..3865835 43734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14974.43 Da        Isoelectric Point: 7.8921

>T87647 WP_032433387.1 NZ_CP024429:c3850244-3849828 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALHLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLTLEDWVI

Download         Length: 417 bp

>T87647 NZ_CP024429:c3850244-3849828 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATTATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACCGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT87647 WP_001261276.1 NZ_CP024429:c3850471-3850241 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT87647 NZ_CP024429:c3850471-3850241 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S8C081

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References