Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3833044..3833780 Replicon chromosome
Accession NZ_CP024429
Organism Klebsiella pneumoniae strain DA48896

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag BKL84_RS19085 Protein ID WP_032433360.1
Coordinates 3833044..3833526 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag BKL84_RS19090 Protein ID WP_003026799.1
Coordinates 3833514..3833780 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKL84_RS19065 3829593..3830333 + 741 WP_050597964.1 molecular chaperone -
BKL84_RS19070 3830337..3830915 + 579 WP_032432061.1 type 1 fimbrial protein -
BKL84_RS19075 3830927..3831871 + 945 WP_077254249.1 fimbrial protein -
BKL84_RS19080 3832084..3832683 + 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
BKL84_RS19085 3833044..3833526 - 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
BKL84_RS19090 3833514..3833780 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
BKL84_RS19095 3834093..3834656 - 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
BKL84_RS19100 3834666..3835298 - 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
BKL84_RS19105 3835316..3836320 - 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
BKL84_RS19110 3836304..3836966 - 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
BKL84_RS19115 3836995..3838134 - 1140 WP_032433368.1 mannitol dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3822101..3865835 43734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T87646 WP_032433360.1 NZ_CP024429:c3833526-3833044 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T87646 NZ_CP024429:c3833526-3833044 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT87646 WP_003026799.1 NZ_CP024429:c3833780-3833514 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT87646 NZ_CP024429:c3833780-3833514 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References