Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1237517..1238136 Replicon chromosome
Accession NZ_CP024429
Organism Klebsiella pneumoniae strain DA48896

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag BKL84_RS06155 Protein ID WP_002892050.1
Coordinates 1237517..1237735 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag BKL84_RS06160 Protein ID WP_002892066.1
Coordinates 1237762..1238136 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKL84_RS06125 1233560..1233823 + 264 WP_032432663.1 type B 50S ribosomal protein L31 -
BKL84_RS06130 1233823..1233963 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
BKL84_RS06135 1233960..1234658 - 699 WP_023287311.1 GNAT family N-acetyltransferase -
BKL84_RS06140 1234759..1236210 + 1452 WP_004183206.1 PLP-dependent aminotransferase family protein -
BKL84_RS06145 1236185..1236655 - 471 WP_020802585.1 YlaC family protein -
BKL84_RS29635 1236676..1236816 + 141 WP_004147370.1 hypothetical protein -
BKL84_RS06150 1236788..1237354 - 567 WP_002892030.1 maltose O-acetyltransferase -
BKL84_RS06155 1237517..1237735 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
BKL84_RS06160 1237762..1238136 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
BKL84_RS06165 1238622..1241768 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
BKL84_RS06170 1241791..1242984 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T87636 WP_002892050.1 NZ_CP024429:c1237735-1237517 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T87636 NZ_CP024429:c1237735-1237517 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT87636 WP_002892066.1 NZ_CP024429:c1238136-1237762 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT87636 NZ_CP024429:c1238136-1237762 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References