Detailed information of TA system TA087632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 40179..40794
• Replicon: plasmid pTT13-3
• Accession: NZ_CP024425
• Organism: Paracoccus yeei strain TT13

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: PYTT13_RS20575
• Protein ID: WP_099650572.1
• Coordinates: 40179..40577 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: PYTT13_RS20580
• Protein ID: WP_099650573.1
• Coordinates: 40567..40794 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PYTT13_RS20560	35241..35819	+	579	WP_099650569.1	hypothetical protein	-
PYTT13_RS22990	35835..36002	+	168	WP_157767439.1	hypothetical protein	-
PYTT13_RS20565	36227..38866	-	2640	WP_099650570.1	AAA family ATPase	-
PYTT13_RS20570	38863..39975	-	1113	WP_099650571.1	metallophosphoesterase	-
PYTT13_RS20575	40179..40577	-	399	WP_099650572.1	PIN domain-containing protein	Toxin
PYTT13_RS20580	40567..40794	-	228	WP_099650573.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
PYTT13_RS20585	40940..41883	+	944	Protein_36	IS110 family transposase	-
PYTT13_RS20590	41882..42094	+	213	Protein_37	IS3 family transposase	-
PYTT13_RS20600	42167..42814	-	648	WP_099650663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
PYTT13_RS20605	42857..43120	-	264	WP_028720295.1	hypothetical protein	-
PYTT13_RS20610	43285..44290	+	1006	Protein_40	IS3 family transposase	-
PYTT13_RS20615	44578..45582	-	1005	WP_099650574.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..167927	167927

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-114)

Antitoxin

• Antitoxin PemI-like (2-54)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14385.52 Da
• Isoelectric Point: 4.6647

>T87632 WP_099650572.1 NZ_CP024425:c40577-40179 [Paracoccus yeei]
MRADFFDTNIILYLLDDGPKADMAAGLLAHGGVISVQVLNEALVNCIRKAGMSWQEAGDFLAGVRDLCEVIGLSARIHDV
GLAVGERYGFSVYDAMIVSAALTSGCERLYTEDLQHGQLIEGRLRIVNPFRQ

Nucleotide

• Length: 399 bp

>T87632 NZ_CP024425:c40577-40179 [Paracoccus yeei]
ATGCGCGCTGATTTCTTCGACACGAACATCATTCTTTATCTGCTCGACGACGGTCCGAAAGCCGACATGGCTGCCGGTTT
GCTGGCCCATGGCGGTGTGATCAGCGTCCAGGTTCTGAACGAAGCCCTCGTCAACTGCATCAGGAAAGCCGGGATGTCCT
GGCAGGAGGCAGGCGATTTCCTCGCAGGCGTGCGCGACTTGTGCGAGGTGATCGGGCTTTCGGCCAGGATCCATGATGTG
GGCCTGGCTGTCGGTGAGCGATATGGGTTTTCGGTCTATGACGCGATGATCGTCTCGGCTGCGCTGACCAGCGGCTGCGA
GCGGCTCTATACGGAAGATCTTCAGCACGGTCAGCTGATCGAAGGCAGGCTTCGGATCGTCAATCCCTTCCGGCAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8358.61 Da
• Isoelectric Point: 11.3030

>AT87632 WP_099650573.1 NZ_CP024425:c40794-40567 [Paracoccus yeei]
MQIAKWGNSLAVRLPADLVRELGLKAGDEIELHPAGKGFAVRRRPSAEDVLADLRRFRGRLPAAERMSRDDAHAR

Nucleotide

• Length: 228 bp

>AT87632 NZ_CP024425:c40794-40567 [Paracoccus yeei]
ATGCAGATCGCGAAATGGGGCAATTCGCTGGCCGTCCGGTTGCCTGCCGACCTCGTGCGCGAACTGGGGCTGAAGGCTGG
CGACGAGATCGAGCTGCATCCAGCCGGAAAAGGCTTTGCCGTGCGTCGCAGGCCTTCGGCCGAGGATGTGCTTGCCGATC
TGCGCCGATTTCGCGGACGGCTTCCTGCCGCAGAGAGAATGAGCCGCGACGACGCGCATGCGCGCTGA