Detailed information of TA system TA087624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 943164..943723
• Replicon: chromosome
• Accession: NZ_CP024420
• Organism: Brucella suis strain QH05

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: CS875_RS04875
• Protein ID: WP_004688321.1
• Coordinates: 943478..943723 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: CS875_RS04870
• Protein ID: WP_002964097.1
• Coordinates: 943164..943478 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CS875_RS04820	938854..939057	-	204	WP_004690812.1	hypothetical protein	-
CS875_RS04825	939060..939275	-	216	WP_002971459.1	hypothetical protein	-
CS875_RS04830	939272..939475	-	204	WP_002964088.1	hypothetical protein	-
CS875_RS04835	939525..940244	+	720	WP_006072696.1	hypothetical protein	-
CS875_RS04840	940241..940471	+	231	WP_002964091.1	hypothetical protein	-
CS875_RS04845	940468..940743	+	276	WP_002964092.1	hypothetical protein	-
CS875_RS04850	940766..941353	+	588	WP_004683738.1	hypothetical protein	-
CS875_RS04855	941588..942298	+	711	WP_004683739.1	porin family protein	-
CS875_RS04860	942646..942816	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
CS875_RS04870	943164..943478	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
CS875_RS04875	943478..943723	-	246	WP_004688321.1	BrnT family toxin	Toxin
CS875_RS04880	943856..944494	+	639	Protein_891	tRNA-guanine transglycosylase	-
CS875_RS04885	944564..945321	+	758	WP_099686914.1	IS5 family transposase	-
CS875_RS04890	945322..946095	+	774	WP_006132649.1	hypothetical protein	-
CS875_RS04895	946230..947876	+	1647	WP_004689673.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	937842..951192	13350

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T87624 WP_004688321.1 NZ_CP024420:c943723-943478 [Brucella suis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T87624 NZ_CP024420:c943723-943478 [Brucella suis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT87624 WP_002964097.1 NZ_CP024420:c943478-943164 [Brucella suis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT87624 NZ_CP024420:c943478-943164 [Brucella suis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA