Detailed information of TA system TA087623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 27045..27633
• Replicon: plasmid pA388
• Accession: NZ_CP024419
• Organism: Acinetobacter baumannii strain A388

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A3G6Z3M6
• Locus tag: A388_RS19705
• Protein ID: WP_000438826.1
• Coordinates: 27045..27332 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: N9M5I3
• Locus tag: A388_RS19710
• Protein ID: WP_001983304.1
• Coordinates: 27319..27633 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A388_RS19675	22513..22806	+	294	WP_000930834.1	helix-turn-helix domain-containing protein	-
A388_RS19680	22796..23698	+	903	WP_000431810.1	reverse transcriptase family protein	-
A388_RS19685	23834..25408	+	1575	WP_046889990.1	type I restriction-modification system subunit M	-
A388_RS19690	25408..25668	+	261	Protein_32	restriction endonuclease subunit S	-
A388_RS19695	25732..26436	+	705	WP_001067856.1	IS6-like element IS26 family transposase	-
A388_RS19700	26488..26739	-	252	Protein_34	hypothetical protein	-
A388_RS19705	27045..27332	+	288	WP_000438826.1	BrnT family toxin	Toxin
A388_RS19710	27319..27633	+	315	WP_001983304.1	BrnA antitoxin family protein	Antitoxin
A388_RS19715	27761..30172	+	2412	WP_000932953.1	TonB-dependent receptor ZnuD2	-
A388_RS19720	30477..30938	+	462	WP_000761346.1	DIP1984 family protein	-
A388_RS19935	31261..31434	-	174	WP_001282484.1	hypothetical protein	-
A388_RS19725	31434..31805	-	372	WP_000504218.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-Ia / blaOXA-58	-	1..33036	33036
	-	flank	IS/Tn	-	-	25732..26436	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11303.72 Da
• Isoelectric Point: 6.0889

>T87623 WP_000438826.1 NZ_CP024419:27045-27332 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T87623 NZ_CP024419:27045-27332 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12208.84 Da
• Isoelectric Point: 7.2026

>AT87623 WP_001983304.1 NZ_CP024419:27319-27633 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT87623 NZ_CP024419:27319-27633 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A3G6Z3M6

Antitoxin

• AlphaFold DB: N9M5I3