Detailed information of TA system TA087615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 782213..782841
• Replicon: chromosome
• Accession: NZ_CP024412
• Organism: Glaesserella parasuis strain SH0104

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CRH16_RS04160
• Protein ID: WP_112062882.1
• Coordinates: 782659..782841 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CRH16_RS04155
• Protein ID: WP_010786740.1
• Coordinates: 782213..782626 (-)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRH16_RS04125	778133..778804	-	672	WP_010786731.1	helix-turn-helix transcriptional regulator	-
CRH16_RS04130	778931..779131	+	201	WP_112062880.1	helix-turn-helix domain-containing protein	-
CRH16_RS04135	779467..780192	+	726	WP_041603233.1	Rha family transcriptional regulator	-
CRH16_RS04140	780189..781019	+	831	WP_021114965.1	hypothetical protein	-
CRH16_RS04145	781019..781693	+	675	WP_112062881.1	holin	-
CRH16_RS04150	781751..782179	+	429	Protein_769	recombination protein NinB	-
CRH16_RS04155	782213..782626	-	414	WP_010786740.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CRH16_RS04160	782659..782841	-	183	WP_112062882.1	type II toxin-antitoxin system HicA family toxin	Toxin
CRH16_RS04165	783087..783656	+	570	WP_112062883.1	recombination protein NinG	-
CRH16_RS04170	783653..784030	+	378	WP_112062884.1	antitermination protein	-
CRH16_RS04175	784039..784257	-	219	WP_012621837.1	hypothetical protein	-
CRH16_RS11640	784554..784730	+	177	WP_005714781.1	hypothetical protein	-
CRH16_RS04180	785179..785454	+	276	WP_201288374.1	hypothetical protein	-
CRH16_RS04185	785429..785974	+	546	WP_112062885.1	lysozyme	-
CRH16_RS04190	785947..786291	+	345	WP_160580396.1	DUF2570 family protein	-
CRH16_RS04205	786785..787252	+	468	WP_005714379.1	DUF1441 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	767237..843605	76368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-130)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6784.98 Da
• Isoelectric Point: 10.7209

>T87615 WP_112062882.1 NZ_CP024412:c782841-782659 [Glaesserella parasuis]
VDSKTIIKMIEADGWKFHHATGSHHHFTHPIKKGIVTVIHPKKDLKKGTEHSILKQVGLK

Nucleotide

• Length: 183 bp

>T87615 NZ_CP024412:c782841-782659 [Glaesserella parasuis]
GTGGATAGCAAAACCATAATTAAAATGATTGAAGCGGACGGTTGGAAATTTCACCATGCAACAGGAAGTCACCACCACTT
CACTCACCCAATCAAAAAAGGCATTGTTACTGTCATTCATCCCAAAAAGGATTTAAAAAAAGGAACAGAACATTCAATAC
TAAAACAAGTGGGGCTAAAATAG

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15280.41 Da
• Isoelectric Point: 4.4693

>AT87615 WP_010786740.1 NZ_CP024412:c782626-782213 [Glaesserella parasuis]
MLYPIAIEPGDETHAFGVVVPDIPGCFSAGDTLEEAYSNAKEAILGHLELLVELGEEIPMPTSIDNHRKNPDFAEYDFFF
GFVDVDISHLLGKAERINITLPSYLIKRIDDFVAVHKEYKNRSNFLAKIAADKILTA

Nucleotide

• Length: 414 bp

>AT87615 NZ_CP024412:c782626-782213 [Glaesserella parasuis]
ATGTTATATCCAATTGCAATCGAACCAGGCGATGAAACCCACGCATTTGGCGTTGTTGTGCCTGATATTCCAGGCTGTTT
CTCTGCCGGCGATACGCTTGAAGAAGCCTACAGCAACGCAAAAGAAGCGATTCTTGGTCATCTTGAGTTATTAGTTGAAC
TTGGCGAAGAAATTCCGATGCCAACCTCCATTGATAACCACCGCAAAAACCCTGATTTTGCAGAATATGACTTCTTTTTC
GGCTTTGTTGATGTTGATATTAGCCATTTACTCGGCAAAGCCGAACGCATCAACATCACCCTCCCTAGCTATCTCATCAA
ACGCATTGATGATTTTGTTGCCGTTCACAAAGAGTATAAAAACCGTAGCAACTTCTTAGCTAAAATCGCAGCGGATAAAA
TCTTAACCGCTTAA