Detailed information of TA system TA087614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 699782..700559
• Replicon: chromosome
• Accession: NZ_CP024412
• Organism: Glaesserella parasuis strain SH0104

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: -
• Locus tag: CRH16_RS03720
• Protein ID: WP_021115013.1
• Coordinates: 699782..700282 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: U4SPD7
• Locus tag: CRH16_RS03725
• Protein ID: WP_005710653.1
• Coordinates: 700272..700559 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRH16_RS03685	694907..695062	-	156	WP_005710668.1	YoaH family protein	-
CRH16_RS03690	695080..695808	-	729	WP_021115014.1	tRNA pseudouridine(65) synthase TruC	-
CRH16_RS03695	695805..696119	-	315	WP_005710664.1	YqcC family protein	-
CRH16_RS03700	696161..697075	-	915	WP_112062863.1	N-acetylmuramic acid 6-phosphate etherase	-
CRH16_RS03705	697087..698202	-	1116	WP_082259326.1	anhydro-N-acetylmuramic acid kinase	-
CRH16_RS03710	698357..698692	-	336	WP_005710658.1	outer membrane protein assembly factor BamE	-
CRH16_RS03715	698753..699754	-	1002	WP_005710656.1	nucleoid-associated protein YejK	-
CRH16_RS03720	699782..700282	-	501	WP_021115013.1	GNAT family N-acetyltransferase	Toxin
CRH16_RS03725	700272..700559	-	288	WP_005710653.1	DUF1778 domain-containing protein	Antitoxin
CRH16_RS03730	700705..701094	+	390	WP_016527795.1	RidA family protein	-
CRH16_RS03735	701098..701835	+	738	WP_021112465.1	peptidoglycan editing factor PgeF	-
CRH16_RS03740	702010..703587	+	1578	WP_112062864.1	FAD-binding protein	-
CRH16_RS03745	703691..704191	+	501	WP_075605420.1	CvpA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (4-87)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 19055.23 Da
• Isoelectric Point: 9.3198

>T87614 WP_021115013.1 NZ_CP024412:c700282-699782 [Glaesserella parasuis]
MKSKFIEEPLTKHHQREAFDCGNLEMNRFFKQYARQSHEKGTSKTYISRHLDSQQIIGFYTITLSALDQQHLPELCKKRF
GYHPIPLFTLARLAVDIRYQKQGIGGMLLVKALKRCAIVAEQVGGIGLLIEAKDEDISRWYQSYGAIPLENMPLTLILLF
DTIKGL

Nucleotide

• Length: 501 bp

>T87614 NZ_CP024412:c700282-699782 [Glaesserella parasuis]
ATGAAGAGTAAGTTTATTGAAGAGCCTTTAACGAAGCATCATCAACGTGAAGCCTTTGATTGCGGAAATCTAGAGATGAA
CCGATTTTTTAAACAATACGCTCGGCAATCTCACGAAAAAGGGACATCAAAAACTTACATTAGCCGACACTTAGACAGTC
AACAGATTATCGGATTTTATACAATCACCTTAAGTGCATTAGACCAGCAACACTTACCCGAACTATGCAAAAAACGTTTT
GGCTACCACCCTATTCCGCTCTTTACATTAGCAAGACTTGCAGTGGATATTCGCTATCAAAAACAAGGCATTGGCGGAAT
GCTGTTAGTTAAAGCTCTCAAACGCTGTGCCATCGTTGCCGAACAAGTCGGGGGAATTGGGCTTCTCATTGAAGCAAAAG
ATGAAGACATTTCTCGTTGGTATCAATCTTATGGTGCTATTCCATTGGAAAATATGCCATTAACCTTAATTCTACTCTTT
GATACAATCAAAGGACTTTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10917.47 Da
• Isoelectric Point: 6.4650

>AT87614 WP_005710653.1 NZ_CP024412:c700559-700272 [Glaesserella parasuis]
MPQIANALNDRMSIRISTEDKQTLIRAVALTHTNITEFVLQRILPSAREIIQQYDNRQFSPQDLSLIMELLDNPPKANTK
LQQAAKLAKESYHEE

Nucleotide

• Length: 288 bp

>AT87614 NZ_CP024412:c700559-700272 [Glaesserella parasuis]
ATGCCACAAATTGCAAATGCCCTTAACGATAGAATGTCTATTCGCATTAGTACGGAAGACAAGCAGACTTTAATTAGAGC
CGTTGCACTTACCCATACGAATATTACGGAATTTGTATTACAACGTATTTTGCCTAGTGCCAGAGAAATTATTCAGCAGT
ATGATAATCGGCAATTTTCACCGCAAGATCTCTCGTTGATTATGGAATTGCTTGATAATCCGCCGAAAGCTAATACAAAA
CTACAACAAGCGGCGAAATTAGCAAAGGAAAGCTATCATGAAGAGTAA

Structures

Antitoxin

• AlphaFold DB: U4SPD7