Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2366910..2367475 Replicon chromosome
Accession NZ_CP024315
Organism Rhodococcus ruber strain P14

Toxin (Protein)


Gene name mazF Uniprot ID A0A385HTA4
Locus tag CS378_RS10545 Protein ID WP_010593326.1
Coordinates 2366910..2367266 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A385HSN3
Locus tag CS378_RS10550 Protein ID WP_010593327.1
Coordinates 2367263..2367475 (-) Length 71 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CS378_RS10520 2363124..2363621 - 498 WP_010593321.1 hypothetical protein -
CS378_RS10525 2363985..2365043 - 1059 WP_010593322.1 mannan endo-1,4-beta-mannosidase -
CS378_RS10530 2365230..2365478 + 249 WP_029539937.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
CS378_RS10535 2365475..2365855 + 381 WP_043804893.1 type II toxin-antitoxin system VapC family toxin -
CS378_RS10540 2366008..2366847 + 840 WP_010593325.1 hypothetical protein -
CS378_RS10545 2366910..2367266 - 357 WP_010593326.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CS378_RS10550 2367263..2367475 - 213 WP_010593327.1 ribbon-helix-helix protein, CopG family Antitoxin
CS378_RS10555 2367686..2368267 + 582 WP_010593328.1 GNAT family N-acetyltransferase -
CS378_RS10560 2368275..2368670 - 396 Protein_2089 hypothetical protein -
CS378_RS10565 2368794..2370077 + 1284 WP_029539938.1 competence/damage-inducible protein A -
CS378_RS10570 2370522..2371433 + 912 WP_029539939.1 formylglycine-generating enzyme family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2354062..2367475 13413


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-114)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-40)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12867.56 Da        Isoelectric Point: 7.3229

>T87613 WP_010593326.1 NZ_CP024315:c2367266-2366910 [Rhodococcus ruber]
MIRRAQIHWVDPDPAQGSEAAERRPAVVVSNDRANATAENLGRGVVTVVPLTTNTSHVFPFQVLVEPDESGLTRAAKAQA
EQVRSVSVRRLDTEPVGRLGARTLAALEEALRLHLDLR

Download         Length: 357 bp

>T87613 NZ_CP024315:c2367266-2366910 [Rhodococcus ruber]
ATGATCCGCCGAGCGCAGATCCACTGGGTCGATCCGGACCCGGCGCAGGGTAGCGAAGCGGCCGAGCGCCGGCCGGCAGT
GGTCGTGAGCAACGACCGCGCCAACGCGACCGCCGAGAATCTGGGCAGAGGAGTGGTCACCGTCGTTCCTCTGACCACCA
ACACCTCACACGTCTTTCCGTTCCAGGTACTCGTGGAACCCGACGAGTCAGGGCTGACCAGAGCAGCGAAAGCTCAGGCA
GAACAGGTACGTTCGGTGTCCGTGCGTCGCCTGGACACCGAACCTGTCGGAAGGCTCGGGGCGAGAACTCTCGCAGCGCT
CGAGGAAGCGCTGCGGCTACATCTCGATTTGCGTTGA

Antitoxin


Download         Length: 71 a.a.        Molecular weight: 7652.40 Da        Isoelectric Point: 4.1694

>AT87613 WP_010593327.1 NZ_CP024315:c2367475-2367263 [Rhodococcus ruber]
MTVKFTISLPDEDAHVLDEYIRDHGLRGRSAGVRAAVRLLAAAGLERDYATAFAEVDTDDAWDVTTADTE

Download         Length: 213 bp

>AT87613 NZ_CP024315:c2367475-2367263 [Rhodococcus ruber]
ATGACCGTCAAGTTCACGATCAGCCTCCCGGACGAGGATGCCCACGTCCTCGACGAGTACATCCGGGATCATGGGCTCCG
CGGCCGGTCGGCGGGAGTCCGGGCCGCTGTCCGCCTGCTCGCGGCAGCCGGACTCGAACGGGACTACGCCACAGCCTTTG
CCGAGGTCGACACAGACGACGCGTGGGATGTCACCACGGCCGACACCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A385HTA4

Antitoxin

Source ID Structure
AlphaFold DB A0A385HSN3

References