Detailed information of TA system TA087609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 3694333..3694880
• Replicon: chromosome
• Accession: NZ_CP024311
• Organism: Rhizobium sp. NXC24

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: NXC24_RS18120
• Protein ID: WP_104825235.1
• Coordinates: 3694333..3694572 (+)
• Length: 80 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: NXC24_RS18125
• Protein ID: WP_104824556.1
• Coordinates: 3694566..3694880 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NXC24_RS18090	3689963..3691081	-	1119	WP_199773586.1	D-alanyl-D-alanine carboxypeptidase	-
NXC24_RS18095	3691346..3692506	+	1161	WP_104824552.1	amidohydrolase	-
NXC24_RS18110	3693084..3693437	+	354	WP_104824554.1	hypothetical protein	-
NXC24_RS18115	3693448..3694113	-	666	WP_104824555.1	hypothetical protein	-
NXC24_RS18120	3694333..3694572	+	240	WP_104825235.1	BrnT family toxin	Toxin
NXC24_RS18125	3694566..3694880	+	315	WP_104824556.1	BrnA antitoxin family protein	Antitoxin
NXC24_RS18130	3694959..3695471	+	513	WP_104825236.1	flagellar protein FlaR	-
NXC24_RS18135	3695521..3695787	-	267	WP_104824557.1	BON domain-containing protein	-
NXC24_RS18140	3695991..3697184	+	1194	WP_104824558.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
NXC24_RS18145	3697223..3698137	+	915	WP_104824559.1	pyridoxal-phosphate dependent enzyme	-
NXC24_RS18150	3698298..3698927	+	630	WP_104824560.1	LysE family translocator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (2-73)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-101)

Sequences

Toxin

Protein

• Length: 80 a.a.
• Molecular weight: 8628.03 Da
• Isoelectric Point: 7.0120

>T87609 WP_104825235.1 NZ_CP024311:3694333-3694572 [Rhizobium sp. NXC24]
MDELKRETNLAKHGLDFADLDIEFFAGSVVVPANDGRSMAIGELDGQIVAAVVFKPLGSEAISVISMRPASRKKRKLLW

Nucleotide

• Length: 240 bp

>T87609 NZ_CP024311:3694333-3694572 [Rhizobium sp. NXC24]
CTGGACGAACTGAAGCGCGAAACAAACCTCGCCAAGCACGGCCTGGACTTTGCCGATCTCGATATTGAATTCTTCGCAGG
TTCTGTCGTCGTCCCCGCGAATGATGGCCGCTCCATGGCCATCGGTGAACTGGACGGACAGATCGTTGCAGCCGTCGTAT
TCAAGCCGCTTGGCTCAGAAGCCATCTCGGTCATTTCGATGCGCCCTGCAAGCCGCAAGAAAAGGAAGCTCTTATGGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11661.36 Da
• Isoelectric Point: 10.1854

>AT87609 WP_104824556.1 NZ_CP024311:3694566-3694880 [Rhizobium sp. NXC24]
MVTKFSSRRPLNEREEAEIQKMIASDPDAPEATDEQLAQAKPFAEAFPDLMGSIKRARGRPKVDAPKVAVTLRIDPDVLE
RFQSKGKDWRSDMTHALRKAAGLK

Nucleotide

• Length: 315 bp

>AT87609 NZ_CP024311:3694566-3694880 [Rhizobium sp. NXC24]
ATGGTGACGAAGTTCTCTTCACGGCGCCCTCTGAATGAGAGAGAAGAAGCCGAAATTCAAAAGATGATCGCGTCCGATCC
AGATGCTCCGGAAGCAACGGATGAGCAACTTGCTCAGGCGAAACCCTTCGCAGAAGCCTTTCCGGATCTGATGGGTAGCA
TTAAGCGCGCCAGGGGGCGGCCAAAAGTGGATGCACCGAAAGTCGCCGTCACCCTGCGAATAGATCCGGATGTCCTTGAG
CGATTCCAGTCCAAGGGAAAGGATTGGCGAAGTGATATGACGCATGCGCTACGCAAGGCTGCTGGGCTCAAATAA