Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 13488..14013 Replicon plasmid unnamed2
Accession NZ_CP024291
Organism Escherichia coli O178:H19 strain 2012C-4431

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag A5960_RS26770 Protein ID WP_001159871.1
Coordinates 13488..13793 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag A5960_RS26775 Protein ID WP_000813639.1
Coordinates 13795..14013 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A5960_RS26735 8755..10025 + 1271 Protein_15 translesion error-prone DNA polymerase V subunit UmuC -
A5960_RS26745 10030..10422 - 393 WP_000340836.1 plasmid partitioning/stability family protein -
A5960_RS26750 10427..11398 - 972 WP_001103695.1 hypothetical protein -
A5960_RS26755 11627..12271 + 645 WP_028985392.1 AAA family ATPase -
A5960_RS26760 12265..12540 + 276 WP_000239527.1 hypothetical protein -
A5960_RS26765 12678..13487 - 810 WP_000016958.1 site-specific integrase -
A5960_RS26770 13488..13793 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
A5960_RS26775 13795..14013 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
A5960_RS26780 14609..14869 + 261 WP_000343084.1 hypothetical protein -
A5960_RS26785 14866..15456 + 591 WP_000194564.1 hypothetical protein -
A5960_RS26790 15474..15821 - 348 WP_000142428.1 hypothetical protein -
A5960_RS26800 16006..16284 + 279 WP_001365566.1 hypothetical protein -
A5960_RS26805 16291..16626 - 336 Protein_27 colicin -
A5960_RS26810 16721..17452 - 732 WP_000850413.1 complement resistance protein TraT -
A5960_RS28595 17729..17839 - 111 Protein_29 IS481 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyB / hlyA / hlyC / espP 1..111697 111697


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T87592 WP_001159871.1 NZ_CP024291:c13793-13488 [Escherichia coli O178:H19]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T87592 NZ_CP024291:c13793-13488 [Escherichia coli O178:H19]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCTGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT87592 WP_000813639.1 NZ_CP024291:c14013-13795 [Escherichia coli O178:H19]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT87592 NZ_CP024291:c14013-13795 [Escherichia coli O178:H19]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References