Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4891951..4892605 Replicon chromosome
Accession NZ_CP024289
Organism Escherichia coli O178:H19 strain 2012C-4431

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag A5960_RS25530 Protein ID WP_000244781.1
Coordinates 4892198..4892605 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag A5960_RS25525 Protein ID WP_000354046.1
Coordinates 4891951..4892217 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A5960_RS25500 4887120..4887863 + 744 WP_000951961.1 SDR family oxidoreductase -
A5960_RS25505 4887920..4889353 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
A5960_RS25510 4889398..4889709 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
A5960_RS25515 4889873..4890532 + 660 WP_000250274.1 hemolysin III family protein -
A5960_RS25520 4890728..4891708 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
A5960_RS25525 4891951..4892217 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
A5960_RS25530 4892198..4892605 + 408 WP_000244781.1 protein YgfX Toxin
A5960_RS25535 4892645..4893166 - 522 WP_001055874.1 flavodoxin FldB -
A5960_RS25540 4893278..4894174 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
A5960_RS25545 4894199..4894909 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
A5960_RS25550 4894915..4896648 + 1734 WP_000813218.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T87590 WP_000244781.1 NZ_CP024289:4892198-4892605 [Escherichia coli O178:H19]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T87590 NZ_CP024289:4892198-4892605 [Escherichia coli O178:H19]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT87590 WP_000354046.1 NZ_CP024289:4891951-4892217 [Escherichia coli O178:H19]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT87590 NZ_CP024289:4891951-4892217 [Escherichia coli O178:H19]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References