Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4238070..4238870 Replicon chromosome
Accession NZ_CP024289
Organism Escherichia coli O178:H19 strain 2012C-4431

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag A5960_RS22000 Protein ID WP_000342449.1
Coordinates 4238343..4238870 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag A5960_RS21995 Protein ID WP_001277108.1
Coordinates 4238070..4238336 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A5960_RS21970 4233727..4234395 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
A5960_RS21975 4234388..4235446 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
A5960_RS21980 4235691..4236545 + 855 WP_096096097.1 RNA polymerase sigma factor RpoH -
A5960_RS21990 4236817..4237920 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
A5960_RS21995 4238070..4238336 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
A5960_RS22000 4238343..4238870 + 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
A5960_RS22005 4238867..4239250 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
A5960_RS22015 4239674..4240783 + 1110 WP_001352761.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
A5960_RS22020 4240831..4241757 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
A5960_RS22025 4241754..4243031 + 1278 WP_000803798.1 branched chain amino acid ABC transporter permease LivM -
A5960_RS22030 4243028..4243795 + 768 WP_099528437.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T87587 WP_000342449.1 NZ_CP024289:4238343-4238870 [Escherichia coli O178:H19]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T87587 NZ_CP024289:4238343-4238870 [Escherichia coli O178:H19]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT87587 WP_001277108.1 NZ_CP024289:4238070-4238336 [Escherichia coli O178:H19]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT87587 NZ_CP024289:4238070-4238336 [Escherichia coli O178:H19]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References