Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3422209..3422706 Replicon chromosome
Accession NZ_CP024289
Organism Escherichia coli O178:H19 strain 2012C-4431

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag A5960_RS17885 Protein ID WP_001173343.1
Coordinates 3422209..3422382 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag A5960_RS17890 Protein ID WP_000398619.1
Coordinates 3422410..3422706 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A5960_RS17875 3418879..3420336 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
A5960_RS17880 3420573..3422090 + 1518 WP_001295087.1 lysine--tRNA ligase -
A5960_RS17885 3422209..3422382 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
A5960_RS17890 3422410..3422706 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
A5960_RS17900 3422933..3423205 - 273 WP_000405647.1 N-acetyltransferase -
A5960_RS17905 3423217..3423447 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
A5960_RS17910 3423628..3425259 + 1632 WP_001216486.1 sensor histidine kinase -
A5960_RS17915 3425256..3425975 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T87585 WP_001173343.1 NZ_CP024289:c3422382-3422209 [Escherichia coli O178:H19]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T87585 NZ_CP024289:c3422382-3422209 [Escherichia coli O178:H19]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT87585 WP_000398619.1 NZ_CP024289:c3422706-3422410 [Escherichia coli O178:H19]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT87585 NZ_CP024289:c3422706-3422410 [Escherichia coli O178:H19]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References