Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 3318999..3319594 Replicon chromosome
Accession NZ_CP024289
Organism Escherichia coli O178:H19 strain 2012C-4431

Toxin (Protein)


Gene name chpB Uniprot ID A0A3L3YDL1
Locus tag A5960_RS17335 Protein ID WP_000239576.1
Coordinates 3318999..3319349 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag A5960_RS17340 Protein ID WP_001223208.1
Coordinates 3319343..3319594 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A5960_RS17310 3314275..3315297 - 1023 WP_024251350.1 ABC transporter permease -
A5960_RS17315 3315311..3316813 - 1503 WP_000205810.1 sugar ABC transporter ATP-binding protein -
A5960_RS17325 3317123..3318079 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
A5960_RS17330 3318389..3318919 + 531 WP_000055075.1 inorganic diphosphatase -
A5960_RS17335 3318999..3319349 - 351 WP_000239576.1 endoribonuclease toxin ChpB Toxin
A5960_RS17340 3319343..3319594 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
A5960_RS17345 3319806..3320147 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
A5960_RS17350 3320150..3323929 - 3780 WP_000060901.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12566.53 Da        Isoelectric Point: 6.2232

>T87584 WP_000239576.1 NZ_CP024289:c3319349-3318999 [Escherichia coli O178:H19]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPIRQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T87584 NZ_CP024289:c3319349-3318999 [Escherichia coli O178:H19]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTAGGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT87584 WP_001223208.1 NZ_CP024289:c3319594-3319343 [Escherichia coli O178:H19]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT87584 NZ_CP024289:c3319594-3319343 [Escherichia coli O178:H19]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3L3YDL1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References