Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | ypjF-yeeU/CbtA-CbeA |
| Location | 3243091..3243869 | Replicon | chromosome |
| Accession | NZ_CP024289 | ||
| Organism | Escherichia coli O178:H19 strain 2012C-4431 | ||
Toxin (Protein)
| Gene name | ypjF | Uniprot ID | A0A3U1V6G9 |
| Locus tag | A5960_RS16975 | Protein ID | WP_033805147.1 |
| Coordinates | 3243091..3243465 (-) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A3U1V6I4 |
| Locus tag | A5960_RS16980 | Protein ID | WP_033805146.1 |
| Coordinates | 3243504..3243869 (-) | Length | 122 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| A5960_RS16960 | 3239249..3239662 | + | 414 | WP_001368082.1 | hypothetical protein | - |
| A5960_RS16965 | 3239659..3241128 | + | 1470 | WP_001022619.1 | serine/threonine protein kinase | - |
| A5960_RS16970 | 3241330..3242594 | - | 1265 | Protein_3116 | tyrosine-type recombinase/integrase | - |
| A5960_RS16975 | 3243091..3243465 | - | 375 | WP_033805147.1 | TA system toxin CbtA family protein | Toxin |
| A5960_RS16980 | 3243504..3243869 | - | 366 | WP_033805146.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| A5960_RS16985 | 3243895..3244116 | - | 222 | WP_033805145.1 | DUF987 domain-containing protein | - |
| A5960_RS16990 | 3244113..3244655 | - | 543 | WP_033805144.1 | DNA repair protein RadC | - |
| A5960_RS16995 | 3244668..3245108 | - | 441 | WP_033805143.1 | antirestriction protein | - |
| A5960_RS17000 | 3245139..3245960 | - | 822 | WP_047082063.1 | DUF945 domain-containing protein | - |
| A5960_RS17005 | 3246062..3246292 | - | 231 | WP_033805141.1 | DUF905 domain-containing protein | - |
| A5960_RS17010 | 3246364..3246813 | - | 450 | WP_033805140.1 | hypothetical protein | - |
| A5960_RS17015 | 3246810..3247262 | - | 453 | WP_033805139.1 | hypothetical protein | - |
| A5960_RS17020 | 3247299..3247868 | - | 570 | WP_033805138.1 | hypothetical protein | - |
| A5960_RS17025 | 3247868..3248572 | - | 705 | WP_033805137.1 | WYL domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | fimC / fimI / fimA / fimE / fimB | 3212701..3253983 | 41282 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 13843.88 Da Isoelectric Point: 7.9537
>T87583 WP_033805147.1 NZ_CP024289:c3243465-3243091 [Escherichia coli O178:H19]
MQTISSHLTRAAQPCLSPVEIWQLLLTHLLSQHYGLTLNDTPFSDETTIQKHIDAGISLSDAVNFLVEKYELIRIDRDGC
SVMAQKSPFITSIDILRARKASGLMKRSSHKTVTRATAGQHQEL
MQTISSHLTRAAQPCLSPVEIWQLLLTHLLSQHYGLTLNDTPFSDETTIQKHIDAGISLSDAVNFLVEKYELIRIDRDGC
SVMAQKSPFITSIDILRARKASGLMKRSSHKTVTRATAGQHQEL
Download Length: 375 bp
>T87583 NZ_CP024289:c3243465-3243091 [Escherichia coli O178:H19]
ATGCAAACCATATCATCACACCTGACACGGGCGGCTCAGCCCTGCCTGTCACCCGTTGAAATCTGGCAGTTGCTGCTGAC
TCATCTGTTATCGCAGCACTATGGCCTGACGCTGAATGACACACCGTTCAGTGATGAAACCACCATCCAGAAACATATTG
ATGCAGGAATATCGCTCAGCGATGCGGTGAACTTTCTTGTCGAAAAATATGAACTGATAAGAATTGACCGCGATGGTTGC
TCTGTCATGGCGCAGAAGTCACCGTTTATCACCAGTATCGATATTCTACGTGCCCGGAAAGCCTCCGGTCTGATGAAACG
CAGCAGTCACAAAACTGTTACCAGGGCAACGGCAGGCCAACATCAGGAGCTATAA
ATGCAAACCATATCATCACACCTGACACGGGCGGCTCAGCCCTGCCTGTCACCCGTTGAAATCTGGCAGTTGCTGCTGAC
TCATCTGTTATCGCAGCACTATGGCCTGACGCTGAATGACACACCGTTCAGTGATGAAACCACCATCCAGAAACATATTG
ATGCAGGAATATCGCTCAGCGATGCGGTGAACTTTCTTGTCGAAAAATATGAACTGATAAGAATTGACCGCGATGGTTGC
TCTGTCATGGCGCAGAAGTCACCGTTTATCACCAGTATCGATATTCTACGTGCCCGGAAAGCCTCCGGTCTGATGAAACG
CAGCAGTCACAAAACTGTTACCAGGGCAACGGCAGGCCAACATCAGGAGCTATAA
Antitoxin
Download Length: 122 a.a. Molecular weight: 13393.06 Da Isoelectric Point: 5.0700
>AT87583 WP_033805146.1 NZ_CP024289:c3243869-3243504 [Escherichia coli O178:H19]
MNNHSESGAIPENPPCQQWGLKSTITPCFGARLVQEGDRLHFLADRAGFNGAFSDEHALRLDQAFPLILKQLELMLTSGE
LNPRHPHSVTLYHNGLTCEADTLGSCGYVFIAIYPEQTEPQ
MNNHSESGAIPENPPCQQWGLKSTITPCFGARLVQEGDRLHFLADRAGFNGAFSDEHALRLDQAFPLILKQLELMLTSGE
LNPRHPHSVTLYHNGLTCEADTLGSCGYVFIAIYPEQTEPQ
Download Length: 366 bp
>AT87583 NZ_CP024289:c3243869-3243504 [Escherichia coli O178:H19]
ATGAACAATCACTCAGAATCCGGTGCCATACCGGAGAATCCCCCCTGCCAGCAGTGGGGGCTAAAGAGTACGATCACACC
CTGTTTTGGAGCTCGGCTGGTGCAAGAAGGCGACCGCCTGCATTTTCTCGCTGACCGCGCCGGGTTTAACGGTGCTTTCA
GCGACGAGCATGCGTTGCGCCTAGACCAGGCCTTTCCGTTGATACTCAAGCAGCTGGAGTTGATGCTCACCAGCGGAGAG
CTCAACCCTAGGCACCCGCACAGCGTCACGCTTTACCACAACGGACTCACCTGCGAAGCCGACACACTCGGCTCCTGTGG
CTATGTCTTCATTGCTATCTATCCTGAGCAGACTGAGCCGCAGTAA
ATGAACAATCACTCAGAATCCGGTGCCATACCGGAGAATCCCCCCTGCCAGCAGTGGGGGCTAAAGAGTACGATCACACC
CTGTTTTGGAGCTCGGCTGGTGCAAGAAGGCGACCGCCTGCATTTTCTCGCTGACCGCGCCGGGTTTAACGGTGCTTTCA
GCGACGAGCATGCGTTGCGCCTAGACCAGGCCTTTCCGTTGATACTCAAGCAGCTGGAGTTGATGCTCACCAGCGGAGAG
CTCAACCCTAGGCACCCGCACAGCGTCACGCTTTACCACAACGGACTCACCTGCGAAGCCGACACACTCGGCTCCTGTGG
CTATGTCTTCATTGCTATCTATCCTGAGCAGACTGAGCCGCAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U1V6G9 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U1V6I4 |