Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2585931..2586549 Replicon chromosome
Accession NZ_CP024289
Organism Escherichia coli O178:H19 strain 2012C-4431

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag A5960_RS13710 Protein ID WP_001291435.1
Coordinates 2586331..2586549 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag A5960_RS13705 Protein ID WP_000344800.1
Coordinates 2585931..2586305 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A5960_RS13695 2581020..2582213 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
A5960_RS13700 2582236..2585385 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
A5960_RS13705 2585931..2586305 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
A5960_RS13710 2586331..2586549 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
A5960_RS13715 2586721..2587272 + 552 WP_000102557.1 maltose O-acetyltransferase -
A5960_RS13720 2587388..2587858 + 471 WP_000136192.1 YlaC family protein -
A5960_RS13725 2588022..2589572 + 1551 WP_099528459.1 cyclic-guanylate-specific phosphodiesterase PdeB -
A5960_RS13730 2589614..2589967 - 354 WP_000878141.1 DUF1428 family protein -
A5960_RS13740 2590346..2590657 + 312 WP_000409911.1 MGMT family protein -
A5960_RS13745 2590688..2591260 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T87580 WP_001291435.1 NZ_CP024289:2586331-2586549 [Escherichia coli O178:H19]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T87580 NZ_CP024289:2586331-2586549 [Escherichia coli O178:H19]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT87580 WP_000344800.1 NZ_CP024289:2585931-2586305 [Escherichia coli O178:H19]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT87580 NZ_CP024289:2585931-2586305 [Escherichia coli O178:H19]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References