Detailed information of TA system TA087579

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1843360..1843838
• Replicon: chromosome
• Accession: NZ_CP024289
• Organism: Escherichia coli O178:H19 strain 2012C-4431

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: -
• Locus tag: A5960_RS09860
• Protein ID: WP_032351442.1
• Coordinates: 1843551..1843838 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: -
• Locus tag: A5960_RS09855
• Protein ID: WP_044705396.1
• Coordinates: 1843360..1843551 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5960_RS28465	1838722..1838898	-	177	WP_000753060.1	hypothetical protein	-
A5960_RS09810	1838891..1839073	-	183	WP_001224668.1	hypothetical protein	-
A5960_RS09815	1839221..1839526	-	306	WP_072095369.1	DUF4406 domain-containing protein	-
A5960_RS09820	1839523..1839804	-	282	WP_072094476.1	DNA-binding protein	-
A5960_RS09825	1839837..1840553	-	717	WP_047082289.1	DUF1627 domain-containing protein	-
A5960_RS28180	1840587..1841129	-	543	WP_072130322.1	replication protein	-
A5960_RS28185	1841041..1842072	-	1032	WP_028985607.1	phage replisome organiser	-
A5960_RS09840	1842141..1842566	-	426	WP_000693925.1	Rha family transcriptional regulator	-
A5960_RS09845	1842550..1842822	-	273	WP_000887454.1	helix-turn-helix domain-containing protein	-
A5960_RS09850	1842931..1843332	+	402	WP_000986593.1	helix-turn-helix domain-containing protein	-
A5960_RS09855	1843360..1843551	+	192	WP_044705396.1	hypothetical protein	Antitoxin
A5960_RS09860	1843551..1843838	+	288	WP_032351442.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A5960_RS09865	1844113..1844265	+	153	WP_047082292.1	DUF1391 family protein	-
A5960_RS28190	1844421..1844678	+	258	WP_122993601.1	hypothetical protein	-
A5960_RS09875	1844759..1844944	-	186	WP_000389971.1	hypothetical protein	-
A5960_RS09885	1845491..1845679	+	189	WP_044808604.1	cell division inhibitor	-
A5960_RS09890	1845676..1845864	+	189	WP_000092782.1	DUF1482 family protein	-
A5960_RS09895	1845960..1848389	+	2430	WP_062865829.1	exonuclease	-
A5960_RS09900	1848451..1848720	+	270	WP_000003742.1	excisionase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	stx2B / stx2A	1788534..1856976	68442

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10962.84 Da
• Isoelectric Point: 10.1360

>T87579 WP_032351442.1 NZ_CP024289:1843551-1843838 [Escherichia coli O178:H19]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHARRQFPFSI

Nucleotide

• Length: 288 bp

>T87579 NZ_CP024289:1843551-1843838 [Escherichia coli O178:H19]
ATGTTGCCCATTTTATGGCTACCATCTGCTCGCGATGATTTGCGTCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCGCGTAGACTTAAAATACGTATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACCTATATCCACCAAGCG
AACGGGTTTCTGGGTTGAGAGAGATAGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATGCTCGCCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7031.85 Da
• Isoelectric Point: 4.0270

>AT87579 WP_044705396.1 NZ_CP024289:1843360-1843551 [Escherichia coli O178:H19]
MSTALSPIVSEFETIEQENSYNEWLRAKVASSLADPRPAIPHDEVMAEMENLIAQIAVTNRSE

Nucleotide

• Length: 192 bp

>AT87579 NZ_CP024289:1843360-1843551 [Escherichia coli O178:H19]
ATGAGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTATCGAACAAGAAAACAGTTACAACGAATGGTTGCGCGC
TAAAGTAGCGTCAAGCCTTGCAGACCCTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAGGAGCGAGTAA